溶瘤病毒M1诱导宫颈癌细胞C-33A凋亡的作用及机制
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篇名: 溶瘤病毒M1诱导宫颈癌细胞C-33A凋亡的作用及机制
TITLE: Effects and Mechanism of Oncolytic Virus M 1 Inducing the Apoptosis of Cervical Cancer C- 33A Cells
摘要: 目的:研究溶瘤病毒M1(简称“M1病毒”)诱导宫颈癌细胞C-33A凋亡的作用及机制。方法:采用MTT法检测不同滴度(0、0.001、0.01、0.1、1、10PFU/cell)M1病毒处理后C-33A细胞的存活率;将C-33A细胞分为对照组(0PFU/cell)和M1病毒低、中、高剂量组(0.001、0.01、0.1PFU/cell),加入相应滴度病毒,培养48h后,采用流式细胞仪检测细胞的凋亡率和感染率,采用Westernblot法检测细胞中C/EBP同源蛋白(CHOP)、胱天蛋白酶12(caspase-12)、caspase-3、活化的caspase-3(cleaved-caspase-3)的表达水平。结果:经不同滴度M1病毒处理后,C-33A细胞存活率均显著降低(P<0.01),且呈剂量依赖趋势。与对照组比较,M1病毒各剂量组细胞的凋亡率和感染率以及中、高剂量组细胞中CHOP、caspase-12、cleaved-caspase-3(中剂量组除外)蛋白的表达水平均显著升高(P<0.01)。结论:M1病毒可诱导宫颈癌细胞C-33A的凋亡,其作用机制可能与激活内质网应激通路有关。
ABSTRACT: OBJECTIVE:To study the effects and mechanism of oncolytic virus M 1(called M 1 virus for short )inducing the apoptosis of cervical cancer C-33A cells. METHODS :MTT assay was used to detect survival rate of C- 33A cells that were treated with different titers (0,0.001,0.01,0.1,1,10 PFU/cell)of M 1 virus. C- 33A cells were divided into control group (0 PFU/cell), low-dose,medium-dose and high-dose groups of M 1 virus(0.001,0.01,0.1 PFU/cell). After treated with corresponding titers of M1 virus for 48 h,flow cytometry was used to detect the apoptotic rate and infection rate of cells;Western blot was performed to detect the protein expression of C/EBP homologous proteins (CHOP),caspase-12,caspase-3 and cleaved-caspase- 3. RESULTS : After treated with different titers of M 1 virus,the survival rate of C- 33A cells decreased significantly (P<0.01),and showed a dose-dependent tr end. Compared with control group ,the apoptotic rate and infection rate of cells in M 1 virus groups as well as the protein expression of CHOP ,caspase-12 and cleaved-caspase- 3(except for medium-dose group )in M 1 virus medium-dose and high-dose groups were increased significantly (P<0.01). CONCLUSIONS :M1 virus can induce the apoptosis of cervical cancer C-33A cells ,and its mechanism may be related to the activation of endoplasmic reticulum stress pathway.
期刊: 2021年第32卷第23期
作者: 肖晓,周雅思,彭楚茵,邓金清,王来友,朱文博
AUTHORS: XIAO Xiao,ZHOU Yasi,PENG Chuyin ,DENG Jinqing ,WANG Laiyou ,ZHU Wenbo
关键字: 溶瘤病毒M1;宫颈癌;C-33A细胞;内质网应激;细胞凋亡
KEYWORDS: Oncolytic virus M 1;Cervical cancer ;C-33A cells ;Endoplasmic reticulum stress ;Cell apoptosis
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