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藏药秦艽花的质量标准提升研究
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| 篇名： | 藏药秦艽花的质量标准提升研究 |
| TITLE： | Study on the improvement of quality standard for Tibetan medicine Qinjiaohua |
| 摘要： | 目的 提升藏药秦艽花的质量标准，为全面评价其质量提供科学依据。方法采用显微及薄层色谱（TLC）法对16批不同产地、不同基原的秦艽花药材进行定性分析；参照2020年版《中国药典》（四部）通则中相应方法，分别进行水分、总灰分、酸不溶性灰分、醇溶性浸出物含量的测定；建立高效液相色谱（HPLC）法，同时测定秦艽花中5种成分（马钱苷酸、獐牙菜苦苷、龙胆苦苷、獐牙菜苷、异荭草苷）的含量。结果秦艽花药材粉末呈浅棕黄色；粉末显微特征清晰，可见花粉粒、导管、非腺毛、花冠表皮细胞和花萼表皮细胞等。TLC鉴别结果显示，在供试品色谱图中，与对照品（异荭草苷）色谱相应位置上显相同颜色的荧光斑点。16批药材的水分、总灰分、酸不溶性灰分、醇溶性浸出物的含量分别为5.40％～8.87％、3.76％～6.40％、0.27％～0.58％、26.81％～42.51％。含量测定方法学考察结果均符合药典要求；16批秦艽花样品中马钱苷酸、獐牙菜苦苷、龙胆苦苷、獐牙菜苷、异荭草苷的含量分别为3.13～9.36、1.26～22.39、13.80～74.60、1.24～12.22、2.58～14.96mg/g。结论本研究在秦艽花已有质量标准的基础上增加了显微鉴别、TLC鉴别、含量测定以及水分、总灰分、酸不溶性灰分和醇溶性浸出物等检查项，并初步拟定秦艽花中水分不得过9.0％、总灰分不得过6.5％、酸不溶性灰分不得过0.6％、醇溶性浸出物不得少于26.0％、龙胆苦苷的含量不得低于13.8mg/g。 |
| ABSTRACT： | OBJECTIVE To improve the quality standard of T ibetan medicine of Qinjiaohua ，and to provide scientific basis for comprehensive quality evaluation. METHODS The qualitative analysis of 16 batches of Qinjiaohua with different producing areas and different origins was carried out by microscopic and TLC identification. According to the method stated in 2020 edition of Chinese Pharmacopoeia ，water content ，total ash content ，acid-insoluble ash content and alcohol-soluble extract content were determined. HPLC method was used to determine the contents of 5 components （loganic acid ，swertiamarin，gentiopicrin， swertionolin，isoorientin） in Qinjiaohua. RESULTS The medicinal powder of Qinjiaohua was light brown-yellow ，and the microscopic features of the powder were clear ，and pollen grains ，ducts，non-glandular hairs ，corolla epidermal cells and calyx epidermal cells were all found. The results of TLC indentification showed that there were fluorescent spots of the same color in the chromatogram of the tested product and the corresponding position of substance control （isoorientin）. The content ranges of water content，total ash content ，acid-insoluble ash content and alcohol-soluble extract were 5.40％-8.87％，3.76％-6.40％，0.27％-0.58％， 26.81％-42.51％，respectively. The results of content determination methodology met the requirements of pharmacopoeia ；the content ranges of loganic acid ，swertiamarin，gentiopicrin，swertionolin and isoorientin in 16 batches of Qinjiaohua were 3.13-9.36，1.26-22.39，13.80-74.60，1.24-12.22，2.58-14.96 mg/g，respectively. CONCLUSIONS On the basis of the original quality standard of Qinjiaohua ，microscopic identification ，TLC identification ，content determination and examination items of water，total ash ，acid-insoluble ash and alcohol-soluble extract are added. It is preliminarily proposed that water content ，total ash content and acid-insoluble ash content should not exceed 9.0％，6.5％ and 0.6％，while the contents of ethanol-soluble extract and gentiopicrin should not be less than 26.0％ and 13.8 mg/g，respectively. |
| 期刊： | 2022年第33卷第01期 |
| 作者： | 钟镥，罗世英，张静，张艺 |
| AUTHORS： | ZHONG Lu，LUO Shiying ，ZHANG Jing，ZHANG Yi |
| 关键字： | 秦艽花；质量标准；显微鉴别；薄层色谱法；高效液相色谱法；含量测定 |
| KEYWORDS： | Qinjiaohua；quality standard ；microscopic identification ；TLC；HPLC；content determination |
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