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知母栽培品和野生品的指纹图谱建立及差异性成分确认
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| 篇名： | 知母栽培品和野生品的指纹图谱建立及差异性成分确认 |
| TITLE： | Establishment of fingerprints and differential component identification of cultivated and wild Anemarrhena asphodeloides |
| 摘要： | 目的 建立知母栽培品和野生品的指纹图谱，确认两者的差异性成分。方法采用蒸发光散射检测器，以高效液相色谱法结合《中药色谱指纹图谱相似度评价系统（2012版）》建立14批知母栽培品和14批知母野生品的指纹图谱，并进行相似度评价，通过与混合对照品色谱图比对指认共有峰。同法测定知母栽培品和野生品中指认共有峰对应成分的含量，通过主成分分析和正交偏最小二乘法-判别分析确认两者的差异性成分，并比较其含量。结果在28批知母药材中共有10个共有峰，指认出新芒果苷（峰1）、芒果苷（峰2）、异芒果苷（峰3）、知母皂苷BⅡ（峰7）、知母皂苷BⅢ（峰8）、知母皂苷Ⅰ（峰9）、知母皂苷AⅢ（峰10）7个成分；各样品指纹图谱与对照指纹图谱的相似度均不低于0.963。知母栽培品与野生品中7个成分的平均总含量分别为74.18、84.72mg/g，二者比较差异有统计学意义（P＜0.05）。知母栽培品与野生品可被分为两类，差异性成分为新芒果苷、芒果苷、知母皂苷BⅡ、知母皂苷AⅢ（变量投影重要性值均大于1）。知母栽培品中新芒果苷含量明显高于野生品（P＜0.05），芒果苷、知母皂苷BⅡ和知母皂苷AⅢ含量均明显低于野生品（P＜0.05）。结论成功建立了知母的指纹图谱，并初步确认了知母栽培品和野生品的差异性成分。 |
| ABSTRACT： | OBJECTIVE To establish the fingerprints of c ultivated and wild Anemarrhena asphodeloides，and to identify their differential components. METHODS Using an evaporative light-scattering detector ， the high performance liquid chromatography combined with Similarity Evaluation System of TCM Chromatographic Fingerprint （2012 edition） were used to establish fingerprints of 14 batches of cultivated A. asphodeloides and 14 batches of wild medicinal materials ，and evaluate their similarity. The common peaks were identified by comparison with the chromatogram of the mixed control. At the same time ，the contents of components corresponding to common peaks in cultivated and wild A. asphodeloides were determined. The principal component analysis and orthogonal partial least squares discrimination analysis were adopted to identify differential components of them ，and compare the contents of them. RESULTS Among 28 batches of A. asphodeloides ，10 common peaks were found ，i.e. neomangiferin（peak 1），mangiferin（peak 2），isomangiferin（peak 3），timosaponin B Ⅱ（peak 7），timosaponin B Ⅲ（peak 8）， timosaponin Ⅰ（peak 9），timosaponin A Ⅲ（peak 10）. The similarities of fingerprints of samples with control fingerprint were no less than 0.963. The average total contents of seven components in cultivated and wild A. asphodeloides were 74.18 and 84.72 mg/g， respectively；there was statistical significance （P＜0.05）. The cultivated and wild A. asphodeloides could be divided into two categories. The differential components were neomangiferin ，mangiferin，timosaponin B Ⅱ and timosaponin A Ⅲ（VIP values were all higher than 1）. The content of neomangiferin in cultivated products was significantly higher than that in wild products （P＜ 0.05），and the contents of mangiferin ，timosaponin B Ⅱ and ti mosaponin A Ⅲ were significantly lower than those in wild products （P＜0.05）. CONCLUSIONS Fingerprint of A. asphodeloides is established ，and differential components of cultivated and wild A. asphodeloides are identified primarily. |
| 期刊： | 2022年第33卷第02期 |
| 作者： | 常鑫鑫，李倩，薛紫鲸，景松松，赵艳云，郑玉光，宋军娜 |
| AUTHORS： | CHANG Xinxin ，LI Qian，XUE Zijing，JING Songsong ，ZHAO Yanyun ，ZHENG Yuguang ，SONG Junna |
| 关键字： | 知母；野生品；栽培品；指纹图谱；多成分定量分析；差异性成分 |
| KEYWORDS： | Anemarrhenae asphodeloides；wild product ； |
| 阅读数： | 263 次 |
| 本月下载数： | 9 次 |
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