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蛇床子药材指纹图谱及4种香豆素类成分含量测定方法的建立
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| 篇名： | 蛇床子药材指纹图谱及4种香豆素类成分含量测定方法的建立 |
| TITLE： | Establishment of the fingerprint of Cnidium monnieri and a method for the content determination of 4 kinds of coumarins |
| 摘要： | 目的 建立蛇床子药材的指纹图谱及其中4种香豆素类成分的含量测定方法。方法采用超高效液相色谱法，利用《中药色谱指纹图谱相似度评价系统（2012版）》建立21批蛇床子药材的指纹图谱，并进行相似度评价，与对照品比对指认共有峰。以10个共有峰峰面积为变量，采用组间连接法对21批蛇床子药材进行系统聚类分析。采用相同超高效液相色谱法，以蛇床子素为内参物，计算花椒毒素、佛手柑内酯、欧前胡素的相对校正因子，采用一测多评法计算其含量，并与外标法测定结果进行比较。结果21批蛇床子药材的指纹图谱中共标识出10个共有峰，相似度为0.997～1.000；指认出峰4为花椒毒素，峰8为佛手柑内酯，峰9为欧前胡素，峰10为蛇床子素。21批样品被划分为3类，其中S7为一类，S14为一类，其余19批为一类。一测多评法与外标法测定的花椒毒素、佛手柑内酯、欧前胡素含量的相对偏差分别为0.88％～1.07％、2.22％～2.29％、0.67％～2.93％。结论成功建立了蛇床子药材的超高效液相色谱指纹图谱及4种香豆素类成分含量测定的一测多评法。 |
| ABSTRACT： | OBJECTIVE To establi sh the fingerprint of Cnidium monnieri and a method for the content determination of 4 kinds of coumarins. METHODS Ultra-high performance liquid chromatography （UPLC） method was adopted to establish the fingerprints of 21 batches of C. monnieri ； their similarities were evaluated with Similarity Evaluation System of TCM Chromatographic Fingerprint （2012 edition）；common peaks were identification by comparison with reference substance. Using 10 common peak areas as variables ，cluster analysis was performed for 21 batches of C. monnieri by the method of between groups. The relative correction factors of xanthotoxin ，bergapten and imperatorin were calculated by the same UPLC method with osthole as the internal reference. The contents of them were calculated by quantitative analysis of multi-components by single marker （QAMS），and compared with the results of external standard method. RESULTS Totally 10 common peaks were identified in the fingerprints of 21 batches of C. monnieri ；the similarities ranged from 0.997 to 1.000. Peak 4 was identified as xanthotoxin ，peak 8 as bergapten ，peak 9 as imperatorin and peak 10 as osthole. A total of 21 batches of samples were divided into 3 categories，of which S 7 was clustered into one category ，S14 was clustered into one category ，and the other 19 batches were clustered into one category. The relative deviations of the contents of xanthotoxin ，bergapten and imperatorin determined by QAMS and external standard method were in the range of 0.88％ -1.07％ ，2.22％ -2.29％ ，0.67％ -2.93％ ，respectively. CONCLUSIONS UPLC fingerprint of C. monnieri is successfully established ，and QAMS method for content determination of 4 coumarins is also established. |
| 期刊： | 2022年第33卷第02期 |
| 作者： | 潘晓君，吕渭升，杨文惠，罗宇琴，梁月仪，魏梅，孙冬梅，陈向东，霍文杰，李振雨 |
| AUTHORS： | PAN Xiaojun，LYU Weisheng，YANG Wenhui， LUO Yuqin，LIANG Yueyi，WEI Mei，SUN Dongmei，CHEN Xiangdong，HUO Wenjie，LI Zhenyu |
| 关键字： | 蛇床子；超高效液相色谱法；指纹图谱；香豆素类成分；一测多评法 |
| KEYWORDS： | Cnidium monnieri ；ultra-high performance liquid chromatography ；fingerprint；coumarins；quantitative analysis of |
| 阅读数： | 315 次 |
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