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枇杷叶生品及不同炮制品质量评价
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| 篇名： | 枇杷叶生品及不同炮制品质量评价 |
| TITLE： | Quality evaluation of crude drug and different processed products of Eriobotryae Folium |
| 摘要： | 目的 评价枇杷叶生品及不同炮制品的质量。方法将10批枇杷叶生品分别制成蜜炙枇杷叶、姜汁炙枇杷叶、姜汁煮枇杷叶、甘草汁炙枇杷叶、甘草汁煮枇杷叶、清炒枇杷叶，共70批样品。采用热浸法测定其醇溶性浸出物含量，采用紫外-可见分光光度法测定其总三萜酸含量（以熊果酸计），采用高效液相色谱法测定其中野鸦椿酸等5个三萜酸类成分的含量；采用高效液相色谱法联合《中药色谱指纹图谱相似度评价系统（2004A版）》建立指纹图谱并进行相似度评价，与混合对照品比对指认共有峰。采用SPSS22.0软件和SIMCA-P14.1软件进行系统聚类分析、主成分分析、正交偏最小二乘法-判别分析。结果枇杷叶生品、蜜炙枇杷叶、姜汁炙枇杷叶、姜汁煮枇杷叶、甘草汁炙枇杷叶、甘草汁煮枇杷叶、清炒枇杷叶中醇溶性浸出物平均测定值分别为25.90％、39.95％、27.44％、28.20％、28.38％、26.36％、29.26％，总三萜酸平均含量分别为40.62、49.33、52.56、46.38、52.17、55.06、53.41mg/g，野鸦椿酸、山楂酸、科罗索酸、齐墩果酸、熊果酸的平均含量和平均总含量分别为1.966～4.808、1.459～2.824、4.525～8.172、1.294～1.817、6.294～8.470、15.538～25.671mg/g。70批样品中有11个共有峰，指认出峰2、5、6、10、11分别为野鸦椿酸、山楂酸、科罗索酸、齐墩果酸、熊果酸。枇杷叶生品及不同炮制品与生品指纹图谱的相似度为0.919～1.000。70批样品中，10批枇杷叶生品聚为一类，10批姜汁煮枇杷叶聚为一类，其他50批枇杷叶炮制品聚为一类；前2个主成分的累计方差贡献率为80.682％；变量投影重要性（VIP）值排序为峰2（野鸦椿酸）＞峰5（山楂酸）＞峰6（科罗索酸）＞峰9（未知成分）＞峰11（熊果酸）＞峰10（齐墩果酸），其值均大于1。结论枇杷叶生品经炮制后，醇溶性浸出物、总三萜酸含量和5个三萜酸类成分（野鸦椿酸、山楂酸、科罗索酸、齐墩果酸、熊果酸）总含量均有不同程度的增加，其中蜜炙枇杷叶中醇溶性浸出物最高，甘草汁煮枇杷叶中总三萜酸含量最高，姜汁煮枇杷叶中5个三萜酸类成分总含量最高。野鸦椿酸、山楂酸、科罗索酸、熊果酸、齐墩果酸等成分可能是影响枇杷叶生品及炮制品质量的差异性成分。 |
| ABSTRACT： | OBJECTIVE To evalu ate the quality of crude drug and di fferent processed products of Eriobotryae Folium . METHODS Ten batches of Eriobotryae Folium were processed into honey-stir-baked Eriobotryae Folium ，ginger-juice-stir-baked Eriobotryae Folium ，ginger-juice-boiled Eriobotryae Folium ，licorice-juice-stir-baked Eriobotryae Folium ，licorice-juice-boiled Eriobotryae Folium ，stir-fried Eriobotryae Folium ，totally 70 batches of samples . The contents of alcohol-soluble extracts ，the contents of total triterpene acids （calculated by ursolic acid ）and five triterpene acids such as euscaphic acid were determined by hot-dipping method ，ultraviolet and visibe spectrophotometry and high performance liquid chromatography （HPLC），respectively. The fingerprints were established with HPLC and their similarity evaluation was conducted with Similarity Evaluation System of TCM Chromatographic Fingerprint （2004A）. Common peaks were identified by comparison with mixed control. Hierarchical clustering analysis ，principal component analysis and orthogonal partial least squares-discriminant analysis （OPLS-DA） were performed by using SPSS 22.0 software and SIMCA-P 14.1 software. RESULTS In Eriobotryae Folium ，honey-stir-baked Eriobotryae Folium ，ginger-juice-stir-baked Eriobotryae Folium ，ginger-juice-boiled Eriobotryae Folium ，licorice-juice-stir-baked Eriobotryae Folium ，licorice-juice-boiled Eriobotryae Folium ，stir-fried Eriobotryae Folium ，average contents of alcohol-soluble extracts were 25.90％，39.95％，27.44％，28.20％，28.38％，26.36％ and 29.26％；average contents of total triterpene acids were 40.62，49.33，52.56，46.38，52.17，55.06 and 53.41 mg/g；average contents of euscaphic acid ，crataegolic acid ，corosolic acid ， oleanolic acid ，ursolic acid and average total content were 1.966-4.808，1.459-2.824，4.525-8.172，1.294-1.817，6.294-8.470， 15.538-25.671 mg/g，respectively. There were 11 common peaks in 70 batches of samples ，and the peak 2，5，6，10 and 11 were identified as euscaphic acid ，crataegolic acid ，corosolic acid ， oleanolic acid and ursolic acid. The similarities of crude drug different processed products with crude drug fringer print were 0.919-1.000. Among 70 batches of samples ，10 batches of Eriobotryae Folium could be clustered into one category ，and 10 batches of ginger- juice-boiled Eriobotryae Folium could be clustered into one category ；other 50 batches of processed products of Eriobotryae Folium could be clustered into one category ； the cumulative variance contribution rate of the first two principal components was 80.682％；variable importance in projection （VIP）value was in descending order ，i.e. peak 2（euscaphic acid ）＞peak 5（crataegolic acid ）＞peak 6（corosolic acid ）＞peak 9 （unknown component ） ＞peak 11 （ursolic acid ）＞peak 10 （oleanolic acid ）， which of them were all higher than 1. CONCLUSIONS After processing ，the contents of alcohol-soluble extracts ，total triterpene acids and the total content of five triterpene acids （euscaphic acid ，crataegolic acid ，corosolic acid ，oleanolic acid and ursolic acid ）increased in varying degrees ， among which the content of alcohol-soluble extracts in honey-stir-baked Eriobotryae Folium was the highest ，the content of total triterpene acids in licorice-juice-boiled Eriobotryae Folium was the highest ，and total content of five triterpene acids in ginger- juice-boiled Eriobotryae Folium was the highest. Euscaphic acid ，crataegolic acid ，corosolic acid ，ursolic acid ，oleanolic acid and other components may be the differential components affecting the quality of raw and processed the leaves from Eriobotryae Folium . |
| 期刊： | 2022年第33卷第02期 |
| 作者： | 高伟城，王小平，何丽珊 |
| AUTHORS： | GAO Weicheng ，WANG Xiaoping ，HE Lishan |
| 关键字： | 枇杷叶；炮制品；醇溶性浸出物；三萜酸类成分；指纹图谱；化学模式识别；质量评价 |
| KEYWORDS： | Eriobotryae Folium ；processed product ；alcohol-soluble extracts ；triterpene acids ；fingerprint；chemical pattern |
| 阅读数： | 218 次 |
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