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基于指纹图谱和网络药理学的连翘质量标志物预测分析
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| 篇名： | 基于指纹图谱和网络药理学的连翘质量标志物预测分析 |
| TITLE： | Predictive analysis of quality markers of Forsythia suspensa based on fingerprint and network pharmacology |
| 摘要： | 目的 预测连翘的质量标志物（Q-Marker）。方法采用高效液相色谱法建立10批连翘药材的指纹图谱，并进行共有峰指认，筛选连翘药材中的Q-Marker候选成分；采用气相色谱-质谱联用技术（GC-MS）对连翘挥发油成分进行分析，筛选挥发油中的Q-Marker候选成分；然后将筛选的Q-Marker候选成分进行网络药理学分析，构建“连翘Q-Marker候选成分-靶点-通路”网络，并预测连翘的Q-marker。结果与结论10批连翘药材共得到21个共有峰，指认了连翘苷、连翘酯苷A、松脂素-β-D-葡萄糖苷、芦丁4个成分；经GC-MS分析后得到连翘挥发油中β-蒎烯、α-蒎烯、松油烯-4-醇、柠檬烯、γ-松油烯、α-水芹烯、β-月桂烯7个Q-Marker候选成分；进一步经网络药理学分析共得到16个关键靶点和17条通路，初步预测连翘苷、连翘酯苷A、松脂素-β-D-葡萄糖苷、芦丁、松油烯-4-醇、α-水芹烯、α-蒎烯、β-蒎烯为连翘的Q-marker。 |
| ABSTRACT： | OBJ ECTIVE To predict the quality marker （Q-Marker）of Forsythia suspensa . METHODS The fingerprints of 10 batches of F. suspensa were established by high performance liquid chromatography. The common peaks were confirmed. The candidate components of Q-Marker in F. suspensa were screened. The volatile oil of F. suspensa were analyzed by gas chromatography-mass spectrometry （GC-MS），and the candidate components of Q-Marker in volatile oil were screened. The network pharmacology analysis was performed for the candidate components of Q-Marker. The network diagram of the “candidate components of F. suspensa Q-Marker-target-pathway”was constructed to predict the Q-marker of F. suspensa . RESULTS & CONCLUSIONS Twenty-one common peaks were obtained for 10 batches of F. suspensa ，and four components were identified as phillyrin，forsythoside A ，pinoresinol-4-O-β-D-glucoside and rutin. Seven candidate components were obtained by GC-MS analysis，such as β-pinene，α-pinene，terpinen-4-ol，limonene，γ-terpinene，α-phellandrene，β-myrcene. By network pharmacology analysis， 16 key targets and 17 pathways were obtained. It was preliminarily predicted that phillyrin ， forsythoside A ， pinoresinol-4-O-β-D-glucoside，rutin，terpinen-4-ol，α-phellandrene，α-pinene and β-pinene were Q-marker of F. suspensa . |
| 期刊： | 2022年第33卷第03期 |
| 作者： | 景奉堂，冯帅，王静，吕绪桢，张天屹，张天锡，李峰 |
| AUTHORS： | JING Fengtang ，FENG Shuai，WANG Jing，LYU Xuzhen ，ZHANG Tianyi ，ZHANG Tianxi ，LI Feng |
| 关键字： | 连翘；质量标志物；指纹图谱；网络药理学 |
| KEYWORDS： | Forsythia suspensa ；quality marker ；fingerprint；network pharmacology |
| 阅读数： | 217 次 |
| 本月下载数： | 16 次 |
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