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苦杏仁皮中膳食纤维和黄酮同步提取工艺的优化
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| 篇名： | 苦杏仁皮中膳食纤维和黄酮同步提取工艺的优化 |
| TITLE： | Optimization of the simultaneous extraction technology of dietary fiber and flavonoids from the peel of Prunus armeniaca |
| 摘要： | 目的 优化苦杏仁皮中膳食纤维和黄酮的同步提取工艺。方法采用酶-重量法计算膳食纤维含量，采用紫外分光光度法测定黄酮含量；以料液比、pH、木瓜蛋白酶溶液浓度、α-淀粉酶溶液浓度、酶解温度和酶解时间为因素，膳食纤维和黄酮含量为指标，采用单因素实验和正交实验设计优化提取工艺。结果最优提取工艺为料液比1∶10（g/mL）、pH5、木瓜蛋白酶溶液浓度0.5％、α-淀粉酶溶液浓度0.5％、酶解温度50℃、酶解时间1h。经3次实验验证，得膳食纤维的平均含量为0.801g/g（RSD＝1.95％），黄酮的平均含量为2.135mg/g（RSD＝2.44％），平均综合评分为0.988分（RSD＝0.81％）。结论所得最优提取工艺稳定、可行。 |
| ABSTRACT： | OBJEC TIVE To optimize the simultaneous extraction technology of dietary fiber （DF）and flavonoids from the peel of Prunus armeniaca . METHODS The content of DF was calculated with enzyme-gravimetric method ，and the content of flavonoids was determined by ultraviolet spectrophotometry. The orthogonal design and single factor test were used to optimize the extraction technology ，with the factors of liquid-solid ratio ，pH，papain concentration ，α-amylase concentration ，temperature of enzymatic hydrolysis and time of enzymatic hydrolysis as factors ，using the contents of DF and flavonoids as indexes. RESULTS The optimal extraction technology included the solid-liquid ratio of 1 ∶ 10（g/mL），pH5，0.5％ papain and 0.5％ α-amylase， enzymatic hydrolysis at 50 ℃ for 1 h. After three times of validation ，the average content of DF was 0.801 g/g（RSD＝1.95％）， and the average content of flavonoids was 2.135 mg/g（RSD＝2.44％）. The average comprehensive score was 0.988（RSD＝ 0.81％）. CONCLUSIONS The optimal extraction technology is stable and feasible. |
| 期刊： | 2022年第33卷第06期 |
| 作者： | 黄琼连，周敏贤，龙江玲，黄莹莹，杨婷，夏荃 |
| AUTHORS： | HUANG Qionglian ，ZHOU Minxian ，LONG Jiangling ，HUANG Yingying ，YANG Ting，XIA Quan |
| 关键字： | 苦杏仁皮；膳食纤维；黄酮；正交实验设计；同步提取；工艺优化 |
| KEYWORDS： | the peel of Prunus armeniaca ；dietary fiber ；flavonoids；orthogonal design ；simultaneous extracxtion ；technology |
| 阅读数： | 206 次 |
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