基于主要活性成分的白子菜叶干燥方式及采收时间研究
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篇名: 基于主要活性成分的白子菜叶干燥方式及采收时间研究
TITLE: Study on drying methods and harvesting time of Gynura divaricata leaf based on main active constituents
摘要: 目的 测定不同干燥方式及采收时间白子菜叶中主要活性成分的含量,以确定其最优干燥方式及采收时间。方法分别制备不同干燥方式[阴干、晒干、烘干(60、70、80℃)、微波干燥、冷冻干燥]和不同采收时间(3~10月)白子菜叶样品,采用热浸法测定其中水溶性浸出物的含量,采用紫外-可见分光光度法测定其中总黄酮和多糖的含量,采用高效液相色谱法测定其中紫云英苷的含量,采用层次分析法进行综合评价。结果阴干、晒干、60℃烘干、70℃烘干、80℃烘干、微波干燥、冷冻干燥所用时间分别为7d、5d、8h、5h、3.5h、6min、1d,所得白子菜叶中水溶性浸出物的平均含量分别为55.98%、60.78%、52.33%、49.54%、46.87%、59.70%、58.24%,总黄酮分别为3.27%、3.22%、1.99%、1.70%、1.31%、3.92%、2.28%,多糖分别为4.70%、6.09%、6.48%、5.45%、5.74%、5.76%、7.15%,紫云英苷分别为0.48%、0.46%、0.24%、0.23%、0.20%、0.48%、0.29%;微波干燥的综合评分最高,为0.9963分。3~10月采收样品中水溶性浸出物的平均含量分别为41.50%、40.57%、39.16%、40.65%、40.68%、43.30%、45.19%、40.12%,总黄酮分别为2.24%、2.81%、3.87%、3.92%、3.82%、3.93%、3.66%、3.25%,多糖分别为4.41%、4.61%、4.98%、5.26%、5.75%、5.94%、5.32%、4.47%,紫云英苷分别为0.20%、0.21%、0.25%、0.26%、0.25%、0.24%、0.25%、0.21%;5~9月采收样品的综合评分均超过0.92分,且以8月的综合评分最高(0.9886分)。结论微波干燥所得白子菜叶的质量较好,5~9月采收较优。
ABSTRACT: OBJECTIVE To determ ine the contents of main active constituents in Gynura divaricata leaf with different drying methods and at different harvesting time ,so as to confirm the best drying method and harvesting time. METHODS G. divaricata leaf with different drying methods [drying in the shade ,drying in the sun ,oven drying (60℃,70℃,80℃),microwave drying and freeze drying] and different harvesting time (March to October )were prepared. The content of water-soluble extract was determined by hot dip method. The contents of total flavonoids and polysaccharides were determined by ultraviolet-visible spectrophotometry. The content of astragalin was determined by HPLC. Analytic hierarchy process was used for comprehensive analysis. RESULTS The time of drying in the shade ,drying in the sun ,drying at 60 ℃,drying at 70 ℃,drying at 80 ℃, microwave drying and freeze drying were 7 d,5 d,8 h,5 h,3.5 h,6 min and 1 d respectively. The average contents of water-soluble extract in G. divaricata leaf were 55.98%,60.78%,52.33%,49.54%,46.87%,59.70% and 58.24%;those of total flavonoids were 3.27%,3.22%,1.99%,1.70%,1.31%,3.92% and 2.28%;those of polysaccharides were 4.70%,6.09%, 6.48%,5.45%,5.74%,5.76% and 7.15%;those of astragalin were 0.48%,0.46%,0.24%,0.23%,0.20%,0.48%,0.29%. The comprehensive score of microwave drying was the highest ,being 0.996 3. The average contents of water-soluble extract from March to October were 41.50%,40.57%,39.16%,40.65%,40.68%,43.30%,45.19% and 40.12%;those of total flavonoids were 2.24%,2.81%,3.87%,3.92%,3.82%,3.93%,3.66% and 3.25%;those of polysaccharides were 4.41%,4.61%, 4.98%,5.26%,5.75%,5.94%,5.32% and 4.47%;those of astragalin were 0.20%,0.21%,0.25%,0.26%,0.25%,0.24%, 0.25% and 0.21%,respectively. The comprehensive scores of samples collected from May to September exceeded 0.92,and the comprehensive score in August was the highest (0.988 6). CONCLUSIONS Microwave-dried Gynura divaricata leaf has the best quality ,and the best harvesting time is from May to September.
期刊: 2022年第33卷第12期
作者: 武英茹,李媛媛,李宁,郭丁丁,郭福贵,兰子君,赵琳儒,倪艳
AUTHORS: WU Yingru ,LI Yuanyuan ,LI Ning,GUO Dingding ,GUO Fugui,LAN Zijun,ZHAO Linru,NI Yan
关键字: 白子菜叶;干燥方式;采收时间;水溶性浸出物;总黄酮;多糖;紫云英苷;含量测定
KEYWORDS: Gynura divaricata leaf; drying methods ;
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