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地稔中6种成分含量测定及其与抗氧化活性的相关性分析
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| 篇名： | 地稔中6种成分含量测定及其与抗氧化活性的相关性分析 |
| TITLE： | Content determination of 6 components in Melastoma dodecandrum and analysis of its correlation with antioxidant activity |
| 摘要： | 目的 建立同时测定地稔药材中6种成分含量的方法，考察上述成分与抗氧化活性的相关性。方法采用超高效液相色谱法进行测定，以牡荆素为内参物，采用一测多评（QAMS）法计算23批地稔药材中没食子酸、原儿茶酸、异牡荆素、芦丁、鞣花酸的含量，并与外标法测定结果进行比较。采用1，1-二苯基-2-三硝基苯肼（DPPH）自由基法、2，2′-联氮-二（3-乙基-苯并噻唑啉-6-磺酸）二铵盐（ABTS）法和铁离子还原/抗氧化能力（FRAP）法测定23批地稔药材的抗氧化活性，以灰色关联度分析和双变量相关性分析评价地稔中6种成分含量与抗氧化活性的相关性。结果地稔中牡荆素的含量为0.021％～0.182％，没食子酸、原儿茶酸、异牡荆素、芦丁、鞣花酸的QAMS法含量测定结果分别为0.008％～0.042％、0.003％～0.023％、0.071％～0.283％、0.013％～0.140％、0.006％～0.021％，与外标法测定结果比较的P均大于0.05。灰色关联度分析显示，6种成分含量与抗氧化活性的灰色关联系数为0.7276～0.8669。双变量相关性分析显示，牡荆素、异牡荆素、芦丁含量与抗氧化活性呈显著正相关（P＜0.05或P＜0.01），没食子酸、原儿茶酸含量与抗氧化活性呈显著负相关（P＜0.05），鞣花酸与抗氧化活性无显著相关性。结论成功建立了同时测定地稔中6种成分含量的QAMS法；地稔中6种成分含量与抗氧化活性均有较高相关性。 |
| ABSTRACT： | OBJECTIVE To establish a method for simultaneous determi nation of six components in Melastoma dodecandrum and investigate its correlation with antioxidant activity. METHODS Ultra high-performance liquid chromatography （UPLC）method was adopted. The contents of gallic acid ，protocatechuic acid ，isovitexin，rutin and ellagic acid in 23 batches of M. dodecandrum were determined by quantitative analysis of multi-components by single-marker （QAMS）method，using vitexin as the internal reference. Then 1，1-diphenyl-2-picrylhydrazyl（DPPH）free radical method ，2，2′-azino-bis（3-ethylbenzothiazoline-6-sulfonic acid ） diammonium salt （ABTS）method and ferric ion reduction/antioxidant power （FRAP）method were applied to determine the antioxidant activity of 23 batches of M. dodecandrum . Grey correlation analysis and bivariate correlation analysis were used to evaluate the correlation between six components and antioxidant activity. RESULTS The content of vitexin were 0.021％-0.182％. The contents of gallic acid ，protocatechuic acid ，isovitexin，rutin and ellagic acid by QAMS method were 0.008％-0.042％， 0.003％-0.023％，0.071％-0.283％，0.013％-0.140％ and 0.006％-0.021％，respectively. Compared with the results of external standard method ，P was greater than 0.05. Grey correlation analysis showed that the grey correlation coefficients between the contents of six components an d antioxidant activity was 0.727 6- 0.866 9. Bivariate correlation analysis showed that the contents of vitexin ，isovitexin and rutin were positively correlated with antioxidant activity （P＜0.05 or P＜0.01），the content of gallic acid and protocatechuic acid were negatively correlated with antioxidant activity （P＜0.05）. There was no significant correlation between ellagic acid and antioxidant activity.CONCLUSIONS QAMS method is successfully established for the simultaneous determination of six components in M. dodecandrum. The six components in M. dodecandrum are highly correlated with antioxidant activity. |
| 期刊： | 2022年第33卷第16期 |
| 作者： | 刘静，明惠仪，麻秀萍，钱松，杨菁，吴登莉，郭江涛 |
| AUTHORS： | LIU Jing，MING Huiyi，MA Xiuping ，QIAN Song，YANG Jing，WU Dengli ，GUO Jiangtao |
| 关键字： | 地稔；含量测定；一测多评法；抗氧化；相关性 |
| KEYWORDS： | Melastoma dodecandrum；content detemination ；QAMS；antioxidant activity ；correlation |
| 阅读数： | 165 次 |
| 本月下载数： | 3 次 |
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