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指纹图谱结合一测多评法评价文冠木药材的质量
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| 篇名： | 指纹图谱结合一测多评法评价文冠木药材的质量 |
| TITLE： | Application of fingerprint combined with quantitative analysis of multi -components by single marker in quality evaluation of Xanthoceras sorbifolia |
| 摘要： | 目的 建立文冠木药材的指纹图谱和黄酮类成分的含量测定方法，对该药材质量进行评价。方法采用高效液相色谱（HPLC）法，以表没食子儿茶素峰为参照，采用《中药色谱指纹图谱相似度评价系统（2004A版）》绘制11批文冠木药材（编号S1～S11）的指纹图谱，进行相似度评价，确定共有峰，并进行聚类分析和主成分分析。以表没食子儿茶素为参照物，采用一测多评法测定16批文冠木药材（编号S1～S16）中没食子儿茶素、儿茶素、表儿茶素、二氢杨梅素、花旗松素、杨梅素的含量，并与外标一点法、标准曲线法检测结果进行比较。结果11批文冠木药材中共有15个共有峰，相似度分别为0.910～1.000；指认出没食子儿茶素（峰1）、表没食子儿茶素（峰2）、儿茶素（峰3）、表儿茶素（峰5）、二氢杨梅素（峰6）、花旗松素（峰14）、杨梅素（峰15）。聚类分析结果显示，S5～S7、S9为一类，S8、S11为一类，S10为一类，S1～S4为一类。主成分分析结果显示，3个主成分的累计方差贡献率为99.24%；S5～S7为一类，S8～S11一类，S3、S4为一类，S1、S2为一类。除杨梅素和部分批次（S12、S14～S16）儿茶素外，3种含量测定方法测得16批药材中没食子儿茶素、儿茶素（剩余批次）、表儿茶素、二氢杨梅素、花旗松素含量的RSD均小于4%（n＝3）。结论所建HPLC指纹图谱和含量测定方法可用于文冠木药材的质量控制；一测多评法可用于测定其中没食子儿茶素、表儿茶素、二氢杨梅素、花旗松素的含量。 |
| ABSTRACT： | OBJECTIVE To establish the fingerprints of Xanthoceras sorbifolia and determine the contents of flavonoids . METHODS HPLC was adopted . Using epigallocatechin as reference ，the fingerprints of 11 batches（No. S1-S11）of X. sorbifolia were drawn with Similarity Evaluation System of Chromatographic Fingerprints of TCM （2004A edition ）. The similarity evaluation was conducted ，the common peaks were also confirmed . Cluster analysis （CA）and principal component analysis （PCA）were also performed. Epigallocatechin was selected as internal reference ，and quantitative analysis of multi -components by single marker （QAMS）was used to determine the contents of gallocatechin ，catechin，epicatechin，dihydromyricetin，taxifolin and myricetin in 16 batches（No. S1-S16）of X. sorbifolia. The results were compared with the results of one point external standard method and standard curve method . RESULTS There were 15 common peaks in 11 batches of X. sorbifolia，and the similarity of them were 0.910-1.000. A total of 7 common peaks were identified ，i.e. galliccatechin（peak 1），epigallocatechin（peak 2），catechin（peak 3），epicatechin（peak 5），dihydromyricetin（peak 6），taxifolin（peak 14）and myricetin （peak 15）. The results of CA showed that S5-S7 and S 9 were clustered into one category ，S8 and S 11 were clustered into one category ，S10 were clustered into one category，S1-S4 were clustered into one category . The results of PCA showed that accumulative variance contribution rate of 3 principal components was 99.24%；S5-S7 were clustered into one category ，S8-S11 were clustered into one category ，S3 and S 4 were clustered into one category ，S1 and S 2 were clustered into one category . With the exception of myricetin and a partial batches （S12，S14-S16） of catechin ，the RSDs measured by the three methods for galliccatechin ，catechin （remaining batches ）， epicatechin，dihydromyricetin and taxifolin in 16 batches of X. sorbifolia were less than 4% （n＝3）. CONCLUSIONS The established HPLC fingerprint and the method for content determination can be used for the quality control of X. sorbifolia. QAMS method can be used for the content determination of galliccatechin，epicatechin，dihydromyricetin and taxifolin . |
| 期刊： | 2022年第33卷第18期 |
| 作者： | 李珍，杨洋，徐萌杰，金一荻 |
| AUTHORS： | LI Zhen，YANG Yang，XU Mengjie ，JIN Yidi |
| 关键字： | 文冠木；黄酮类成分；指纹图谱；一测多评法；高效液相色谱法 |
| KEYWORDS： | Xanthoceras sorbifolia ；flavonoids； finger- |
| 阅读数： | 191 次 |
| 本月下载数： | 3 次 |
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