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复方麝香雪莲柳酯贴膏中4种挥发性成分含量测定方法的建立
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| 篇名： | 复方麝香雪莲柳酯贴膏中4种挥发性成分含量测定方法的建立 |
| TITLE： | Establishment of a method for the determination of four volatile components in Compound shexiang xuelian liuzhi plaster by GC |
| 摘要： | 目的 建立同时测定复方麝香雪莲柳酯贴膏中樟脑、薄荷脑、冰片、水杨酸甲酯含量的方法。方法以乙酸乙酯回流提取制备供试品溶液，采用气相色谱法测定。以ShimadzuSH-Rtx-Wax毛细管柱为色谱柱，以火焰离子化检测器为检测器，检测器温度、进样口温度均为200℃，载气（氮气）流速为2.0mL/min，分离比为20∶1，进样量为1.0μL。结果樟脑、薄荷脑、冰片（以异龙脑、龙脑总和计）、水杨酸甲酯检测质量浓度的线性范围分别为11.5～230.4、10.6～211.6、11.3～225.5、11.0～219.1μg/mL（r＞0.999），精密度、重复性、稳定性（48h）试验的RSD均小于4%；4种成分的平均加样回收率分别为100.7%、99.7%、98.9%、100.7%（RSD分别为4.3%、2.9%、2.2%、3.7%，n＝9）。2种规格的麝香雪莲柳酯贴膏中樟脑、薄荷脑、冰片、水杨酸甲酯的含量分别为16.8～19.5、4.6～6.0、9.8～11.9、6.9～8.2mg/片（7cm×10cm/片）和8.3～8.6、2.2～2.4、4.7～4.8、3.2～3.6mg/片（5cm×7cm/片）。结论成功建立了同时测定复方麝香雪莲柳酯贴膏中4种挥发性成分含量的方法。 |
| ABSTRACT： | OBJECTIVE To establish a method for simultaneously determining the contents of camphor ，menthol，borneol and methyl salicylate in Compound shexiang xuelian liuzhi plaster . METHODS The test solution was prepared by reflux extraction with ethyl acetate ，and was determined by gas chromatography （GC）. A Shimadzu SH -Rtx-Wax capillary column was used as the chromatographic column ，and a flame ionization detector was used as the detector . The detector temperature and the injector temperature were both set at 200 ℃. The flow rate of carrier gas （nitrogen）was 2.0 mL/min，the separation ratio was 20∶1，and the sample size was 1.0 μL. RESULTS The linear ranges of camphor ，menthol，borneol（calculated by the sum of isoborneol and borneol）and methyl salicylate were 11.5-230.4，10.6-211.6，11.3-225.5，11.0-219.1 μg/mL（r＞0.999）. RSDs of the precision ， repeatability and stability （48 h）tests were all less than 4%. The average recoveries of the four components were 100.7%，99.7%， 98.9% and 100.7%（RSDs were 4.3%，2.9%，2.2%，3.7%，n＝9）. The contents of camphor ，menthol，borneol and methyl salicylate in two specifications of Compound shexiang xuelian liuzhi plaster were 16.8-19.5，4.6-6.0，9.8-11.9，6.9-8.2 mg/piece（7 cm×10 cm/piece），and 8.3-8.6，2.2-2.4，4.7-4.8，3.2-3.6 mg/piece （5 cm×7 cm/piece）. CONCLUSIONS The method is successfully established for simultaneous determination of four volatile components in Compound shexiang xuelian liuzhi plaster . |
| 期刊： | 2022年第33卷第20期 |
| 作者： | 周钢，单莲莲，马方圆，柴冰阳，雷慧兰，陶虹，严华 |
| AUTHORS： | ZHOU Gang，SHAN Lianlian，MA Fangyuan，CHAI Bingyang，LEI Huilan，TAO Hong，YAN Hua |
| 关键字： | 复方麝香雪莲柳酯贴膏；樟脑；薄荷脑；冰片；水杨酸甲酯；气相色谱法；含量测定 |
| KEYWORDS： | Compound shexiang xuelian liuzhi plaster ；camphor；menthol；borneol；methyl salicylate ；gas chromatography ； |
| 阅读数： | 177 次 |
| 本月下载数： | 2 次 |
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