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藤黄健骨胶囊质控成分定量分析方法建立及潜在作用机制预测
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| 篇名： | 藤黄健骨胶囊质控成分定量分析方法建立及潜在作用机制预测 |
| TITLE： | Establishment of quantitative analysis method and prediction of potential mechanism for quality control components of Tenghuang jiangu capsules |
| 摘要： | 目的建立藤黄健骨胶囊质控成分的定量分析方法，并预测质控成分的潜在作用机制。方法采用超高效液相色谱-四极杆-静电场轨道阱高分辨质谱（UHPLC-Q-OrbitrapHRMS）法对藤黄健骨胶囊中的7种质控成分进行定量分析；基于网络药理学方法构建“成分-靶点”网络，并进行京都基因和基因组数据库（KEGG）通路富集以及基因本体（GO）功能富集分析，挖掘潜在关键信号通路。结果20批藤黄健骨胶囊中琥珀酸、金丝桃苷、没食子酸、山柰酚、柚皮苷、柚皮素、原儿茶酸的平均含量分别为520.92、67.67、129.48、4.74、397.45、5.66、376.62μg/g。网络药理学结果显示，7种质控成分的62个关键作用靶点基因包括AKT1、TNF、VEGFA、MMP9、PTGS2等；主要富集于细胞因子受体相互作用、细胞核因子、肿瘤坏死因子、白细胞介素17、类风湿性关节炎、Toll样受体等信号通路，涉及炎症反应、信号转导、蛋白磷酸化等生物过程，胞质、包膜等细胞组分，以及酶活性、能量活性等分子功能。结论所建UHPLC-Q-OrbitrapHRMS法可用于藤黄健骨胶囊质控成分的定量分析；其质控成分可能是通过AKT1、TNF、VEGFA等关键靶点，从而映射到类风湿性关节炎、Toll样受体等骨性疾病相关炎症通路，从而发挥治疗作用。 |
| ABSTRACT： | OBJECTIVE To establish a quantitative analysis method for the quality control components in Tenghuang jiangu capsules， and predict the possible action mechanism of the quality control components. METHODS Seven key quality control components in Tenghuang jiangu capsules were quantitatively analyzed by UHPLC-Q-Orbitrap HRMS. The “component-target” network was constructed based on network pharmacology， and the Kyoto Encyclopedia of Genes and Genomes （KEGG） pathway enrichment analysis and gene ontology （GO） function enrichment analysis were further conducted to find the key signaling pathways. RESULTS The average contents of succinic acid， hyperoside， gallic acid， kaempferol， naringin， naringenin and protocatechuic acid in 20 batches of Tenghuang jiangu capsules were 520.92， 67.67， 129.48， 4.74， 397.45， 5.66 and 376.62 μg/g， respectively. The results of network pharmacology showed that the 62 key target genes of the quality control components of the drug included AKT1， TNF， VEGFA， MMP9， PTGS2， etc. They were mainly enriched in cytokine receptor interaction， nuclear factor， tumor necrosis factor， interleukin 17， rheumatoid arthritis， Toll-like receptor and other signal pathways， involving inflammatory reaction， signal transduction， protein phosphorylation and other biological processes， kytoplasm， cell membrane and other cell components， as well as enzyme activity， energy activity and other molecular functions. CONCLUSIONS The established UHPLC- Q-Orbitrap HRMS method can be used for the quantitative analysis of the quality control components of Tenghuang jiangu capsule. Its quality control components may be mapped to inflammatory pathways related to bone diseases such as rheumatoid arthritis and Toll-like receptors through AKT1， TNF， VEGFA and other key targets， so as to play a therapeutic role. |
| 期刊： | 2022年第33卷第22期 |
| 作者： | 周霖，王肖辉，孙志，薛连平，金建文，武婧，李晓静，郑天元，张晓坚 |
| AUTHORS： | ZHOU Lin，WANG Xiaohui， SUN Zhi，XUE Lianping，JIN Jianwen，WU Jing，LI Xiaojing，ZHENG Tianyuan，ZHANG Xiaojian |
| 关键字： | 藤黄健骨胶囊；质控成分；含量测定；网络药理学；机制 |
| KEYWORDS： | Tenghuang jiangu capsule； quality control |
| 阅读数： | 145 次 |
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