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佛手蒸制前后对大鼠燥性指标及血液代谢的影响
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| 篇名： | 佛手蒸制前后对大鼠燥性指标及血液代谢的影响 |
| TITLE： | Effects of Citrus medica before and after being steamed on dryness indexes and blood metabolism in rats |
| 摘要： | 目的 探究佛手蒸制前后对大鼠燥性相关指标和血液代谢的影响。方法将24只SD大鼠随机分为空白组、生佛手组（2.4g/kg）、制佛手组（2.4g/kg）、枳壳组（阳性对照，1.2g/kg），每组6只。连续给药5周，动态观察大鼠体质量、摄食量、饮水量、尿液量，检测大鼠血清中肾水通道蛋白3（AQP3）含量。采用液相色谱-质谱联用技术对大鼠血清样本进行代谢轮廓分析，通过主成分分析（PCA）、正交偏最小二乘法-判别分析（OPLS-DA）等方法筛选差异代谢物，并将筛选后的差异代谢物映射到KEGG数据库进行通路分析。结果各组大鼠体质量均平缓上升；制佛手组大鼠的摄食量、饮水量均具有高于生佛手组和枳壳组的趋势，但差异无统计学意义（P＞0.05）。给药第21天，与枳壳组比较，制佛手组大鼠尿液量显著升高（P＜0.05），血清AQP3含量显著降低（P＜0.05），且均接近空白组。代谢组学研究发现，生佛手主要影响机体的脂代谢，制佛手主要影响机体的氨基酸代谢；生佛手组和制佛手组之间的差异代谢物涉及半胱氨酸和蛋氨酸代谢，甘氨酸、丝氨酸和苏氨酸代谢，牛磺酸和次牛磺酸代谢等通路。结论制佛手可能通过影响环磷酸腺苷-蛋白激酶A通路缓和生佛手的燥性，血清AQP3含量可作为评价佛手炮制前后燥性变化的指标；生佛手和制佛手对大鼠血清内源性代谢物的影响存在较大差异，尤其在脂质代谢方面。 |
| ABSTRACT： | OBJECTIVE To explore the effects of Citrus medica before and after being steamed on blood metabolism and dryness indexes in rats. METHODS Twenty-four SD rats were randomly divided into blank group， C. medica group （2.4 g/kg）， processed C. medica group （2.4 g/kg）， Citrus aurantium group （positive control， 1.2 g/kg）， with 6 rats in each group. After 5 weeks of continuous administration， the body weight， food intake， water intake and urine volume were observed dynamically. The content of aquaporin 3 （AQP3） in serum was detected. The metabolic profile of rat serum samples was analyzed by liquid chromatography-mass spectrometry. Principal component analysis （PCA） and orthogonal partial least squares-discriminant analysis （OPLS-DA） were used to screen differential metabolites， and screened differential metabolites were mapped to the KEGG database for pathway analysis. RESULTS The body weight of rats in each group increased slowly； the food intake and water intake of processed C. medica group tended to be higher than those in C. medica group and C. aurantium group， but there was no significant difference among those groups （P＞0.05）. On the 21st day of administration， compared with C. aurantium group， the urine volume of processed C. medica group was significantly increased （P＜0.05）， the serum AQP3 content of processed C. medica group was significantly decreased （P＜0.05）， and were close to those of blank group. According to the metabonomic study， C. medica mainly affected the lipids metabolism， and processed C. medica mainly affected the amino acid metabolism. The differential metabolites pathway between C. medica and processed C. medica involved the metabolism of cysteine and methionine， metabolism of glycine， serine and threonine， and metabolism of taurine and hypotaurine. CONCLUSIONS Processed C. medica might relieve the dryness of C. medica by affecting adenosine phosphate-protein kinase A. The content of AQP3 in serum can be used as the 52 evaluation index for the dryness of C. medica before and after processing. The effects of C. medica and processed C. medica on endogenous metabolites in rat serum are quite different，especially in the aspect of lipid metabolism. |
| 期刊： | 2022年第33卷第23期 |
| 作者： | 聶沁馨，张秋霞，汪金玉，伍沛君，贺亚丽，林鸿彬 |
| AUTHORS： | NIE Qinxin，ZHANG Qiuxia，WANG Jinyu，WU Peijun，HE Yali，LIN Hongbin |
| 关键字： | 佛手；蒸制；燥性；差异代谢物；代谢组学 |
| KEYWORDS： | Citrus medica； steaming； dryness； differential |
| 阅读数： | 183 次 |
| 本月下载数： | 3 次 |
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