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红花龙胆药材的UPLC指纹图谱研究与芒果苷含量测定
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| 篇名： | 红花龙胆药材的UPLC指纹图谱研究与芒果苷含量测定 |
| TITLE： | Study on UPLC fingerprint and content determination of mangiferin of Gentiana rhodantha |
| 摘要： | 目的 为红花龙胆药材的质量控制提供参考依据。方法以52批红花龙胆药材为研究对象，采用超高效液相色谱（UPLC）法，以《中药色谱指纹图谱相似度评价系统（2004A版）》对52批药材样品进行相似度评价，并测定药材中芒果苷含量，再进行化学计量学分析[聚类分析、主成分分析（PCA）和正交偏最小二乘法-判别分析（OPLS-DA）]。结果建立了52批红花龙胆药材样品的UPLC指纹图谱，标定了17个共有峰，指认了其中6个，分别为马钱苷酸（1号峰）、新芒果苷（3号峰）、獐牙菜苦苷（5号峰）、当药苷（6号峰）、芒果苷（7号峰）与异荭草苷（9号峰）。52批药材样品的相似度均大于0.9；聚类分析结果显示，S1～S46、S48～S52聚为一类，S47单独为一类；PCA结果显示，前6个主成分的累计方差贡献率为82.928%；OPLS-DA结果显示，獐牙菜苦苷、芒果苷以及4、14、15、16号峰所代表的化学成分是影响红花龙胆药材质量差异的主要标志性成分。52批药材样品中芒果苷的含量在18.2～101.0mg/g之间，大多符合2020年版《中国药典》规定。结论所建立的UPLC指纹图谱和化学计量学分析方法结合芒果苷含量测定方法，能较为全面地评价红花龙胆药材的质量。 |
| ABSTRACT： | OBJECTIVE To provide reference for quality control of Gentiana rhodantha. METHODS Taking 52 batches of G. rhodantha as subject， ultra-high performance liquid chromatography （UPLC） fingerprint was adopted. The similarity of 52 batches of medicinal materials samples was evaluated by the Similarity Evaluation System for Chromatographic Fingerprints of Traditional Chinese Medicine （2004A edition）； the content of mangiferin was determined； chemometric analyses [cluster analysis， principal component analysis （PCA） and orthogonal partial least squares-discriminant analysis （OPLS-DA）] were performed. RESULTS UPLC fingerprints of 52 batches of G. rhodantha were established， 17 common peaks were identified， and 6 of them were identified， which were loganic acid （peak 1）， neomangiferin （peak 3）， swertiamarin （peak 5）， dangyin （peak 6）， mangiferin （peak 7） and isoorientin （peak 9）. The similarities of 52 batches of medicinal materials samples were all greater than 0.9； cluster analysis showed that S1-S46， S48-S52 clustered into one class， and S47 alone； PCA results showed that the cumulative variance contribution rate of the first six principal components was 82.928%； OPLS-DA results showed that the corresponding components of swertiamarin， mangiferin and chemical composition represented by peak 4， 14， 15， 16 were the main iconic components affecting the quality differences of G. rhodantha medicinal materials. The contents of mangiferin in 52 batches of medicinal material samples ranged from 18.2 to 101.0 mg/g， mostly in accordance with 2020 edition of Chinese Pharmacopoeia. CONCLUSIONS The established UPLC fingerprint and chemometric analysis methods combined with content determination method of mangiferin can comprehensively evaluate the quality of G. rhodantha. |
| 期刊： | 2023年第34卷第01期 |
| 作者： | 杨朝堃；徐仕娟；徐文芬；孙庆文；王波；郭江涛；张永萍 |
| AUTHORS： | YANG Chaokun，XU Shijuan，XU Wenfen，SUN Qingwen，WANG Bo，GUO Jiangtao，ZHANG Yongping |
| 关键字： | 红花龙胆；指纹图谱；超高效液相色谱法；聚类分析；主成分分析；正交偏最小二乘法-判别分析；芒果苷 |
| KEYWORDS： | Gentiana rhodantha； fingerprint； ultra-high performance liquid chromatography； cluster analysis； principal |
| 阅读数： | 182 次 |
| 本月下载数： | 10 次 |
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