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不同产地独一味总黄酮的质量评价
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| 篇名： | 不同产地独一味总黄酮的质量评价 |
| TITLE： | Quality evaluation of total flavonoids of Lamiophlomis rotata from different producing areas |
| 摘要： | 目的 评价不同产地独一味总黄酮的质量。方法采用渗漉法提取、聚酰胺柱纯化S1～S15批次不同产地的独一味总黄酮。采用紫外分光光度法测定独一味总黄酮含量，并计算纯度。通过《中药色谱指纹图谱相似度评价系统（2012版）》软件建立15批独一味总黄酮的高效液相色谱指纹图谱和对照指纹图谱，并分析相似度。采用聚类分析、主成分分析、正交偏最小二乘法-判别分析评价15批独一味总黄酮的质量，并分析影响质量的主要成分。结果15批独一味总黄酮纯度为55.82%～94.12%，平均值为77.72%；其指纹图谱中共确定了5个共有峰，3号峰为木犀草苷，与对照指纹图谱的相似度在0.925～1.000之间；聚类分析将S1、S3～S9聚为第1类，为青海省和西藏自治区样品；S14、S15聚为第2类，均为云南省样品；S10～S13聚为第3类，均为四川省样品；S2聚为第4类；主成分分析得到第1类和第4类质量较优；正交偏最小二乘法-判别分析筛选出2、3、5号峰为引起质量差异的主要成分。结论青海省和西藏自治区的独一味总黄酮质量较好。 |
| ABSTRACT： | OBJECTIVE To evaluate the quality of total flavonoids of Lamiophlomis rotata from different producing areas. METHODS Total flavonoids of S1-S15 batches of L. rotata from different producing areas were extracted by percolation and purified by polyamide column. The content of total flavonoids was determined by UV spectrophotometry， and its purity was calculated. HPLC fingerprint chromatograms and control fingerprint of total flavonoids from 15 batches of L. rotata were established with Similarity Evaluation System of Chromatographic Fingerprint of TCM （2012 version）. Their similarities were analyzed. Cluster analysis， principal component analysis and orthogonal partial least squares-discriminant analysis were used to evaluate the quality of total flavonoids from 15 batches of L. rotata， and the main components which affected the quality of total flavonoids were analyzed. RESULTS The purities of total flavonoids from 15 batches of L. rotata were 55.82%-94.12%， with an average value of 77.72%； a total of five common peaks were identified in the fingerprint， and No. 3 peak was luteolin； the similarities between the fingerprint of each batch of samples and the control fingerprint were 0.925-1.000. By cluster analysis， S1 and S3-S9 were clustered into the first class， which were samples from Qinghai Province and Tibet Autonomous Region. S14 and S15 were clustered into the second class， which were samples from Yunnan Province. S10-S13 were clustered into the third class， which were all samples from Sichuan Province. S2 was clustered into the fourth class. The principal component analysis showed that the qualities of samples from the first and fourth classes were better； peaks 2， 3 and 5 were identified as the main components that caused the differences among different batches of samples by orthogonal partial least squares-discriminant analysis. CONCLUSIONS The qualities of total flavonoids of L. rotata from Qinghai Province and Tibet Autonomous Region are better. |
| 期刊： | 2023年第34卷第04期 |
| 作者： | 陈瑞鑫；蒋运斌；陈文莉；康点点；李蕊；蒋桂华 |
| AUTHORS： | CHEN Ruixin，JIANG Yunbin，CHEN Wenli，KANG Diandian，LI Rui，JIANG Guihua |
| 关键字： | 独一味；总黄酮；指纹图谱；高效液相色谱法；化学计量学；质量评价 |
| KEYWORDS： | Lamiophlomis rotata； total flavonoids； |
| 阅读数： | 171 次 |
| 本月下载数： | 1 次 |
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