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半夏地上、地下部分活性成分分析及镇咳作用比较
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| 篇名： | 半夏地上、地下部分活性成分分析及镇咳作用比较 |
| TITLE： | Analysis of active components in aboveground and underground parts of Pinellia ternata and comparison of antitussive effect |
| 摘要： | 目的 研究半夏地上部分（非药用的茎和叶部分）的活性成分和镇咳作用，并与半夏地下部分（药用的地下块茎部分）进行对比，为半夏综合利用和产品开发提供科学依据。方法采用薄层色谱（TLC）法、气相色谱（GC）法、高效液相色谱（HPLC）法和超高效液相色谱串联质谱（UPLC-MS/MS）法对半夏地上和地下部分的氨基酸类、挥发油类、总黄酮类、有机酸类成分进行定性和定量分析；通过浓氨水引咳实验，对半夏地上和地下部分的镇咳作用进行对比研究。结果TLC结果显示，在与半夏对照药材以及精氨酸、丙氨酸、缬氨酸、亮氨酸、芦丁对照品色谱图相应的位置上，半夏地上和地下部分均显相同颜色的斑点；GC分析结果显示，半夏地上和地下部分挥发油特征图谱的相似度为0.767；HPLC和UPLC-MS/MS分析结果显示，与半夏地下部分相比，地上部分中琥珀酸、槲皮素、山柰酚和异鼠李素的含量分别高了0.15%、0.15%、0.09%、0.03%，天门冬氨酸含量低了2.5mg/g。药效结果显示，与模型对照组比较，各给药组大鼠的咳嗽潜伏期均显著延长（P＜0.05），3min内咳嗽次数均显著减少（P＜0.05），且各给药组大鼠3min内咳嗽次数差异无统计学意义（P＞0.05）。结论半夏地上部分的氨基酸类、挥发油类、总黄酮类化合物组成与半夏地下部分相似，琥珀酸、槲皮素、山柰酚、异鼠李素含量高于半夏地下部分，天门冬氨酸含量低于地下部分；半夏地上部分可延长大鼠咳嗽潜伏期，减少咳嗽次数，具有一定的镇咳效果，但作用比地下部分稍弱。 |
| ABSTRACT： | OBJECTIVE To study active components and antitussive effect of aboveground part of Pinellia ternata （non- medicinal stems and leaves）， and compare them with the underground part of P. ternata （medicinal underground tubers）， providing scientific basis for the comprehensive utilization and product development of P. ternata. METHODS TLC， GC， HPLC and UPLC- MS/MS were used for qualitative and quantitative analysis of amino acids， volatile oil， total flavonoids and succinic acid from the aboveground and underground parts of P. ternata. The antitussive effects of the aboveground and underground parts of P. ternata were compared and studied through cough inducing experiment with concentrated ammonia water. RESULTS Results of TLC showed that at the corresponding positions on the chromatograms of the reference substances of P. ternata， and arginine， alanine， valine， leucine and rutin control， the aboveground and underground parts of P. ternata showed spots of the same color. Results of GC showed that the similarity among characteristic chromatograms of volatile oil from aboveground and underground parts of P. ternata was 0.767； results of HPLC and UPLC-MS/MS showed that compared with underground parts of P. ternata， the contents of succinic acid， quercetin， kaempferol and isorhamnetin increased by 0.15%， 0.15%， 0.09% and 0.03%， and aspartate content decreased by 2.5 mg/g. Pharmacodynamics results showed that compared with model control group， the cough incubation period of rats was prolonged significantly in administration groups （P＜0.05）， and the cough frequency within 3 min was significantly decreased （P＜0.05）； there was no statistical significance in the cough frequency within 3 min among administration groups （P＞0.05）. CONCLUSIONS The composition of amino acids， volatile oils and total flavonoids in aboveground part of P. ternata are similar to underground part of P. ternata， while the content of aspartic acid is lower than that in underground part. The aboveground part of P. ternata can prolong the cough incubation period of rats and reduce the number of coughs， which has a certain antitussive effect， but the effect is slightly weaker than that of the underground part. |
| 期刊： | 2023年第34卷第11期 |
| 作者： | 赵丽；徐加兵；陈晓兰；顾庆云；仇雅静；刘明；丁奕竹 |
| AUTHORS： | ZHAO Li，XU Jiabing，CHEN Xiaolan，GU Qingyun，QIU Yajing，LIU Ming，DING Yizhu |
| 关键字： | 半夏；地下部分；地上部分；活性成分；镇咳作用 |
| KEYWORDS： | Pinellia ternata； underground part； aboveground part； active components； antitussive effect |
| 阅读数： | 155 次 |
| 本月下载数： | 2 次 |
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