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灵芝中10种灵芝酸类成分含量一测多评法的建立
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| 篇名： | 灵芝中10种灵芝酸类成分含量一测多评法的建立 |
| TITLE： | Establishment of quantitative analysis of multi-components by single marker for content determination of 10 ganoderic acids in Ganoderma lucidum |
| 摘要： | 目的 建立同时测定灵芝中10种灵芝酸类成分的一测多评法。方法采用高效液相色谱（HPLC）法，以灵芝酸A为内参物，分别计算灵芝酸B、灵芝酸C2、灵芝酸D、灵芝酸F、灵芝酸H、灵芝烯酸A、灵芝烯酸B、灵芝烯酸C、灵芝烯酸D9种成分的相对校正因子，用相对校正因子计算上述灵芝酸类成分的含量，并与外标法测定结果进行比较。结果灵芝酸A、灵芝酸B、灵芝酸C2、灵芝酸D、灵芝酸F、灵芝酸H、灵芝烯酸A、灵芝烯酸B、灵芝烯酸C、灵芝烯酸D的线性范围分别为0.032～3.996、0.040～4.971、0.037～4.568、0.028～3.558、0.033～4.177、0.044～5.440、0.032～3.944、0.040～4.994、0.045～5.593、0.035～4.342mg/mL（R2均不小于0.9992）；精密度、稳定性（24h）、重复性试验的RSD均小于2%；平均加样回收率分别为99.43%、100.25%、98.50%、99.88%、100.59%、99.64%、98.50%、99.40%、99.64%、99.76%（RSD＜2%，n＝6）。灵芝酸B、灵芝酸C2、灵芝酸D、灵芝酸F、灵芝酸H、灵芝烯酸A、灵芝烯酸B、灵芝烯酸C、灵芝烯酸D的相对校正因子平均值分别是1.7885、1.2882、1.1264、1.6985、0.8854、5.4681、4.2109、5.7808、4.2903；不同产地灵芝样品一测多评法所得含量与外标法的相对误差均在±12%之内。结论以灵芝酸A为内参物同时测定灵芝中10种灵芝酸类成分含量的一测多评法是可行的，该方法准确性和重复性良好，可用于灵芝的质量控制。 |
| ABSTRACT： | OBJECTIVE To establish a quantitative analysis of multi-components by single marker （QAMS） method for simultaneous determination of 10 ganoderic acids in Ganoderma lucidum. METHODS Using ganoderic acid A as internal reference， high-performance liquid chromatography （HPLC） method was adopted to calculate relative correction factors of the other 9 components， such as ganoderic acid B， ganoderic acid C2， ganoderic acid D， ganoderic acid F， ganoderic acid H， ganoderenic acid A， ganoderenic acid B， ganoderenic acid C， ganoderenic acid D； the contents of above ganoderic acids were calculated with relative correction factors， and compared with the results of external standard method. RESULTS The linear relationship of ganoderic acid A， ganoderic acid B， ganoderic acid C2， ganoderic acid D， ganoderic acid F， ganoderic acid H， ganoderenic acid A， ganoderenic acid B， ganoderenic acid C and ganoderenic acid D were 0.032-3.996， 0.040-4.971， 0.037-4.568， 0.028-3.558， 0.033-4.177， 0.044-5.440， 0.032-3.944， 0.040-4.994， 0.045-5.593 and 0.035-4.342 mg/mL （all R 2≥0.999 2）， respectively. RSDs of precision， stability （24 h） and reproducibility tests were all lower than 2%. Their average recovery rates were 99.43%， 100.25%， 98.50%， 99.88%， 100.59%， 99.64%， 98.50%， 99.40%， 99.64% and 99.76%， respectively （RSD＜2%， n＝6）. Relative correction factors of ganoderic acid B， ganoderic acid C2， ganoderic acid D， ganoderic acid F， ganoderic acid H， ganoderenic acid A， ganoderenic acid B， ganoderenic acid C and ganoderenic acid D were 1.788 5， 1.288 2， 1.126 4， 1.698 5， 0.885 4， 5.468 1， 4.210 9， 5.780 8， 4.290 3， respectively. Relative errors between the content obtained by QAMS method and external standard method for G. lucidum from different origins were within ±12%. CONCLUSIONS It is feasible that the contents of 10 ganoderic acids are determined simultaneously by QAMS method， using ganoderic acid A as internal reference. This method shows good precision and reproducibility and can be used for the quality control of G. lucidum. |
| 期刊： | 2023年第34卷第14期 |
| 作者： | 罗舒；宋怡；罗霞；江南；余梦瑶；许晓燕 |
| AUTHORS： | LUO Shu，SONG Yi，LUO Xia，JIANG Nan，YU Mengyao，XU Xiaoyan |
| 关键字： | 灵芝酸；灵芝；一测多评法；相对校正因子 |
| KEYWORDS： | ganoderic acid； Ganoderma lucidum； quantitative analysis of multi-components by single marker； relative |
| 阅读数： | 169 次 |
| 本月下载数： | 22 次 |
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