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连翘醇提物对肺癌NCI-H226细胞增殖、迁移及侵袭的影响
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| 篇名： | 连翘醇提物对肺癌NCI-H226细胞增殖、迁移及侵袭的影响 |
| TITLE： | Effects of Forsythia suspensa ethanol extract on the proliferation，migration and invasion of lung cancer cells NCI-H226 |
| 摘要： | 目的 探究连翘醇提物对肺癌NCI-H226细胞增殖、迁移、侵袭的影响。方法以肺癌NCI-H226细胞为研究对象，将细胞分为对照组，连翘醇提物低、中、高浓度组（5、10、20mg/mL），激活剂组[10mg/mL连翘醇提物+0.5μmol/L核因子κB（NF-κB）信号通路激活剂PMA]，抑制剂组（10mg/mL连翘醇提物+10μmol/LNF-κB信号通路抑制剂BAY11-7082），阳性对照组（20μg/mL顺铂），除对照组细胞不作干预外，其余各组细胞加入相应药物培养24h。检测细胞增殖、迁移、侵袭情况，并计算增殖率、迁移率、侵袭细胞数；检测细胞中NF-κBp65、NF-κB抑制蛋白α（IκBα）、磷酸化NF-κBp65（p-NF-κBp65）、磷酸化IκBα（p-IκBα）蛋白表达水平。结果与对照组比较，连翘醇提物各浓度组和阳性对照组细胞的增殖率、迁移率、侵袭细胞数以及细胞中p-IκBα、p-NF-κBp65蛋白表达水平均显著降低（P＜0.05）；与连翘醇提物中浓度组比较，抑制剂组细胞增殖率、迁移率、侵袭细胞数及细胞中上述蛋白表达水平均显著降低（P＜0.05），而激活剂组均显著升高（P＜0.05）。结论连翘醇提物可抑制肺癌NCI-H226细胞增殖、迁移和侵袭，其作用机制可能与抑制NF-κB信号通路有关。 |
| ABSTRACT： | OBJECTIVE To investigate the effects of Forsythia suspensa ethanol extract on the proliferation， migration and invasion of lung cancer cells NCI-H226. METHODS As research objects， lung cancer cells NCI-H226 were divided into control group， F. suspensa ethanol extract low-， medium- and high-concentration groups （5， 10， 20 mg/mL）， activator group [10 mg/mL F. suspensa ethanol extract+0.5 μmol/L nuclear factor kappa B （NF-κB） signaling pathway activator PMA]， inhibitor group （10 mg/mL F. suspensa ethanol extract+10 μmol/L NF-κB signaling pathway inhibitor BAY 11-7082） and positive control group （20 μg/mL cisplatin）. Except for the control group of cells without intervention， all other groups of cells were cultured with corresponding drugs for 24 hours； the proliferation， migration and invasion of cells were all detected， and the proliferation rate， migration rate， and the number of invading cells were also calculated； protein expressions of NF-κB p65， NF-κB inhibitory protein α （IκBα）， phosphorylated NF-κB p65 （p-NF-κB p65） and phosphorylated IκBα （p-IκBα） were determined. RESULTS Compared with control group， the proliferation rate， migration rate， and the number of invading cells as well as the protein expressions of p- IκBα and p-NF-κB p65 were decreased significantly in F. suspensa ethanol extract groups and positive control group （P＜0.05）. Compared with F. suspensa ethanol extract medium-concentration group， the proliferation rate， migration rate， and the number of invading cells as well as above protein expressions were all decreased significantly in inhibitor group （P＜0.05）， while those of activator group were increased significantly （P＜0.05）. CONCLUSIONS F. suspensa ethanol extract can inhibit the proliferation， migration and invasion of lung cancer cells NCI-H226， and the mechanism of which may be related to the inhibition of NF-κB signaling pathway. |
| 期刊： | 2023年第34卷第15期 |
| 作者： | 黄玉龙；陈学武；王芳婷；张晶；陈蔼莹 |
| AUTHORS： | HUANG Yulong，CHEN Xuewu，WANG Fangting，ZHANG Jing，CHEN Aiying |
| 关键字： | 连翘醇提物；肺癌；核因子κB；增殖；迁移；侵袭 |
| KEYWORDS： | Forsythia suspensa ethanol extract； lung cancer； NF-κB； proliferation； migration； invasion |
| 阅读数： | 63 次 |
| 本月下载数： | 3 次 |
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