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基于代谢组学探讨快松饮防治便秘的作用机制
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| 篇名： | 基于代谢组学探讨快松饮防治便秘的作用机制 |
| TITLE： | Investigation on the mechanism of Kuaisong yin in the prevention and treatment of constipation based on metabonomics |
| 摘要： | 目的 探讨快松饮防治便秘的作用机制。方法采用复方地芬诺酯片建立小鼠和大鼠的慢传输型便秘模型。取2批小鼠，分别分为空白组，模型组，阳性对照组（麻仁软胶囊，0.64g/kg），快松饮低、中、高剂量组（3.2、6.4、12.8g/kg），每组10只；通过小鼠肠推进实验、小鼠排便实验考察快松饮防治便秘的效果。取大鼠分为空白组，模型组，阳性对照组（麻仁软胶囊，0.36g/kg），快松饮低、高剂量组（2.4、4.8g/kg），每组7或8只；每天给药1次，连续给药1周后，利用超高效液相色谱-飞行时间质谱技术对大鼠血清、尿液进行代谢组学分析。结果与模型组比较，快松饮高剂量组小鼠墨汁推进率显著升高、5h内排便数量显著增加（P＜0.05），快松饮中、高剂量组小鼠的首次排便时间显著缩短、5h内排便质量显著增加（P＜0.05或P＜0.01）。血清代谢组学筛选得到16个差异代谢物（如脯氨酸、丙酰基肉碱、硬脂酰溶血性磷脂酰胆碱等）和6条代谢通路（如鞘磷脂代谢、精氨酸和脯氨酸代谢、鞘糖脂生物合成-乳糖和新内酯系列等）；尿液代谢组学筛选得到20个差异代谢物（如前列腺素A2、L-缬氨酸、硬脂酰磷脂酰胆碱、鞘磷脂等）和8条代谢通路（如缬氨酸、亮氨酸和异亮氨酸生物合成，鞘磷脂代谢，丙酮酸代谢等）。结论快松饮可通过回调硬脂酰溶血性磷脂酰胆碱、硬脂酰磷脂酰胆碱、前列腺素A2、L-缬氨酸、脯氨酸等差异代谢物以及调节多种氨基酸代谢途径、鞘磷脂代谢通路等来发挥改善便秘的作用。 |
| ABSTRACT： | OBJECTIVE To explore the mechanism of Kuaisong yin in the prevention and treatment of constipation. METHODS Slow transit constipation （STC） model was established with Compound difenoxylate tablet in mice and rats. Two batches of mice were divided into blank group， model group， positive control group （Maren soft capsule， 0.64 g/kg）， Kuaisong yin low-dose， medium-dose and high-dose groups （3.2， 6.4， 12.8 g/kg）， with 10 mice in each group. The effect of Kuaisong yin on constipation in mice was evaluated by intestinal propulsion experiment and defecation experiment. Rats were divided into blank group， model group， positive control group （Maren soft capsule，0.36 g/kg）， Kuaisong yin low-dose and high-dose groups （2.4， 4.8 g/kg）， with 7 or 8 rats in each group. They were given relevant medicine once a day for 1 week. The metabonomics of serum and urine of rats were analyzed by UPLC-Q-TOF-MS/MS technology. RESULTS Compared with model group， the ink propulsion rate and 5 h defecation volume of mice in Kuaisong yin high-dose group were significantly increased （P＜0.05）； the first defecation time of mice in Kuaisong yin medium-dose and high-dose groups was significantly shortened， and the quality of defecation was significantly reduced within 5 h （P＜0.05 or P＜0.01）. Serum metabonomics screened 16 compounds （such as proline， propionylcarnitine， hemolytic phosphatidylcholine， etc.） and 6 metabolic pathways （such as sphingomyelin metabolism， arginine and proline metabolism， sphingolipid biosynthesis-lactose and neolactone series）. Urine metabonomics screened 20 different metabolites （such as prostaglandin A2， L-valine， phosphatidylcholine， sphingomyelin， etc.） and 8 metabolic pathways （such as valine， leucine and isoleucine biosynthesis， sphingomyelin metabolism， pyruvate metabolism， etc.）. CONCLUSIONS Kuaisong yin can play a role in improving constipation by regulating different metabolites such as hemolytic phosphatidylcholine， phosphatidylcholine， prostaglandin A2， L-valine， proline， and regulating metabolic pathways such as multiple amino acid metabolism， sphingomyelin metabolism， etc. |
| 期刊： | 2023年第34卷第17期 |
| 作者： | 于小聪；刘沈林；王泽琨；李丹婷；赵毅萌；陈辰；陈亚军；束雅春 |
| AUTHORS： | YU Xiaocong，LIU Shenlin，WANG Zekun，LI Danting，ZHAO Yimeng，CHEN Chen，CHEN Yajun，SHU Yachun |
| 关键字： | 快松饮；便秘；药效学；代谢组学；慢传输型便秘 |
| KEYWORDS： | Kuaisong yin； constipation； pharmacodynamics； metabolomics； slow transit constipation |
| 阅读数： | 72 次 |
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