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芍药甘草汤缓急止痛有效部位筛选及其入血成分分析
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| 篇名： | 芍药甘草汤缓急止痛有效部位筛选及其入血成分分析 |
| TITLE： | Screening of effective parts for acute and chronic pain relief of Shaoyao gancao decoction and analysis of its blood components |
| 摘要： | 目的 研究芍药甘草汤缓急止痛的药效物质基础。方法通过大鼠离体肠张力实验考察芍药甘草汤全方、芍药甘草汤乙酸乙酯萃取物及其大孔树脂流出部位和大孔树脂90%乙醇洗脱部位（给药质量浓度按生药量计均为13.44g/mL）的解痉效果。通过小鼠醋酸扭体实验评价大孔树脂流出部位和大孔树脂90%乙醇洗脱部位（给药剂量以生药量计均为2.4g/kg）的止痛效果，并检测小鼠血清中肿瘤坏死因子α（TNF-α）、白细胞介素1β（IL-1β）、前列腺素E（2PGE2）、环氧合酶2（COX-2）水平。采用高效液相色谱-飞行时间质谱技术对灌胃大孔树脂90%乙醇洗脱部位后的小鼠血清进行原型成分及代谢产物鉴定。结果大鼠体外实验中，以大孔树脂90%乙醇洗脱部位的解痉效果最好，其小肠张力抑制率显著高于大孔树脂流出部位（P＜0.05），且与芍药甘草汤全方比较差异无统计学意义（P＞0.05）。小鼠醋酸扭体实验中，与模型组比较，大孔树脂90%乙醇洗脱部位组小鼠的扭体次数显著减少（P＜0.05），扭体潜伏期显著延长（P＜0.05），且小鼠血清中COX-2、IL-1β、PGE2、TNF-α水平均显著降低（P＜0.05）。从血清中共鉴定出包括芍药苷、甘草酸等在内的10种原型成分和包括羟基化甘草苷、葡萄糖醛酸化甘草素等在内的22种代谢产物。结论芍药甘草汤缓急止痛的有效部位为乙酸乙酯萃取物的大孔树脂90%乙醇洗脱部位，甘草酸、芍药苷等10种成分可能是其药效物质基础。 |
| ABSTRACT： | OBJECTIVE To study the pharmacological substance basis of Shaoyao gancao decoction for relieving acute and chronic pain. METHODS The antispasmodic effect of Shaoyao gancao decoction， ethyl acetate extract of Shaoyao gancao decoction and its effluent part of macroporous resin and 90% ethanol elution part of macroporous resin （the concentration of 4 drugs was 13.44 g/mL according to crude drug） was observed by in vitro small intestine tension test in rats. The acetic acid writhing test was conducted in mice to evaluate the analgesic effects of macroporous resin efflux site and macroporous resin 90% ethanol elution site （the dosage of 2.4 g/kg according to crude drug）. The levels of tumor necrosis factor-α （TNF-α）， interleukin-1β （IL- 1β）， prostaglandin E2 （PGE2） and cyclooxygenase-2 （COX-2） in serum of mice were detected. The serum prototype and metabolites of mice after intragastric administration of macroporous resin 90% ethanol elution site were identified by high performance liquid chromatogre-time-of-flight mass spectrometry. RESULTS In vitro experiment showed that 90% ethanol eluting part of macroporous resin represented the best antispasmodic effect， and the inhibitory rate of small intestine tension was significantly higher than macroporous resin efflux site of Shaoyao gancao decoction （P＜0.05） without statistical significance， compared with Shaoyao gancao decoction （P＞0.05）. In the acetic acid writhing experiment， compared with model group， the writhing times of mice in the macroporous resin 90% ethanol elution part group were reduced significantly （P＜0.05）， the writhing latency was prolonged significantly （P＜0.05）， and the levels of COX-2， IL-1β， PGE2 and TNF-α in serum were decreased significantly （P＜0.05）. Ten kinds of protoproducts including paeoniflorin and glycyrrhizic acid were identified from serum of mice， and twenty-two kinds of metabolites including hydroxylated glycyrrhizin and glucosylated liquiritin were identified. CONCLUSIONS The effective part of Shaoyao gancao decoction for relieving acute and chronic pain is 90% ethanol elution part prepared by macroporous resin from the ethyl acetate extract. Ten components， including glycyrrhetinic acid and paeoniflorin， may be the basis of its pharmacological substances. |
| 期刊： | 2024年第35卷第15期 |
| 作者： | 解雨欣；杨正清；肖莲莲；朱喻波；赵勉；胡杨；刘陶世；程建明 |
| AUTHORS： | XIE Yuxin， YANG Zhengqing，XIAO Lianlian，ZHU Yubo，ZHAO Mian，HU Yang，LIU Taoshi，CHENG Jianming |
| 关键字： | 芍药甘草汤；缓急止痛；血清药物化学；药效物质基础 |
| KEYWORDS： | Shaoyao gancao decoction； acute and chronic |
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