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土茯苓中8个成分的含量测定及化学计量学分析
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| 篇名： | 土茯苓中8个成分的含量测定及化学计量学分析 |
| TITLE： | Content determination and chemometric analysis of 8 components in Smilax glabra |
| 摘要： | 目的 为土茯苓药材的质量控制提供依据。方法采用超高效液相色谱法同时测定不同产地20批（编号S1～S20）土茯苓中5-O-咖啡酰基莽草酸、新落新妇苷、落新妇苷、新异落新妇苷、异落新妇苷、黄杞苷、白藜芦醇、异黄杞苷的含量。采用化学计量学方法对20批土茯苓样品进行评价，并筛选影响土茯苓质量的差异标志物。结果所测8个成分在各自检测质量浓度范围内线性关系良好（r≥0.9996）；精密度、重复性和稳定性（24h）试验的RSD均小于2.00%（n＝6）；平均加样回收率为97.60%～106.40%，RSD均小于2.00%（n＝6）。聚类分析结果显示，浙江产（S1～S5）和江西产（S6～S10）样品聚为一类，湖南产（S11～S15）样品聚为一类，云南产（S16～S20）样品聚为一类；主成分分析结果显示，前2个主成分可反映土茯苓中8个成分85.60%的信息量；偏最小二乘-判别分析结果显示，5-O-咖啡酰基莽草酸、落新妇苷、异落新妇苷和新落新妇苷的变量重要性投影值均大于1。结论不同产地土茯苓中上述8个成分含量存在差异，5-O-咖啡酰基莽草酸、落新妇苷、异落新妇苷和新落新妇苷可能是影响土茯苓质量的差异标志物。 |
| ABSTRACT： | OBJECTIVE To provide reference for Smilax glabra quality control. METHODS UPLC method was established to simultaneously determine the contents of 5-O-caffeoylshikimic acid， neoastilbin， astilbin， neoisoastilbin， isoastilbin， engelitin， resveratrol and isoengelitin in 20 batches of S. glabra from different areas （No. S1-S20）. The quality evaluation of 20 batches of samples was performed by chemometrics； the differential biomarkers that affected the quality of S. glabra were screened. RESULTS The measured 8 components had good linear relationship within the range of measured concentration （r≥0.999 6）. RSDs of precision， repeatability and stability tests （24 h） were all lower than 2.00% （n＝6）. The average recoveries varied between 97.60% and 106.40% （RSDs were all lower than 2.00%， n＝6）. Cluster analysis showed that the samples produced in Zhejiang （S1-S5） and Jiangxi （S6-S10） were clustered into one category； the samples produced in Hunan （S11-S15） were clustered into one category； the samples produced in Yunnan （S16-S20） were clustered into one category. Principal component analysis showed that the first two principal components could represent 85.60% information of 8 components in S. glabra. Partial least squares-discriminant analysis showed that variable importance projection values of 5-O-caffeoylshikimic acid， astilbin， isoastilbin and neoastilbin were all greater than 1. CONCLUSIONS There are differences in the contents of the above 8 components in S. glabra from different origins； 5-O-caffeoylshikimic acid， astilbin， isoastilbin and neoastilbin may be differential markers affecting the quality of S. glabra. |
| 期刊： | 2024年第35卷第19期 |
| 作者： | 邹惠亮；王新财；陈柯；崔虹；孟令杰；刘百联；陈敏 |
| AUTHORS： | ZOU Huiliang，WANG Xincai，CHEN Ke，CUI Hong，MENG Lingjie，LIU Bailian，CHEN Min |
| 关键字： | 土茯苓；化学计量学；含量测定；超高效液相色谱法；5-O-咖啡酰基莽草酸；落新妇苷；异落新妇苷；新落新妇苷 |
| KEYWORDS： | Smilax glabra； chemometrics； content determination； UPLC； 5-O-caffeoylshikimic acid； astilbin； isoastilbin； |
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