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补阳还五汤对高脂血症模型小鼠红细胞的影响
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| 篇名： | 补阳还五汤对高脂血症模型小鼠红细胞的影响 |
| TITLE： | Effect of Buyang huanwu decoction on red blood cells in hyperlipidemia model mice |
| 摘要： | 目的 研究补阳还五汤对高脂血症模型小鼠红细胞的影响。方法以高脂饲料喂养雄性C57BL/6小鼠制备高脂血症模型。将造模成功的小鼠随机分为模型组、阿托伐他汀钙组（0.26g/kg）、非诺贝特组（1.3mg/kg）和补阳还五汤高、中、低剂量组（18.6、9.3、4.6g/kg），每组10只；另取10只以普通饲料喂养的小鼠作为正常组。各组小鼠灌胃相应药物或生理盐水，每日1次，连续21d。末次给药后，各组小鼠称体重，检测血糖、总胆固醇（TC）、甘油三酯（TAG）、高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇（LDL-C）水平，观察红细胞形态变化，检测红细胞膜中葡萄糖转运蛋白1（GLUT1）、红细胞膜蛋白带4.2（protein4.2）、小窝蛋白1（caveolin-1）、筏蛋白1（flotillin-1）的表达水平。结果与模型组比较，补阳还五汤各剂量组小鼠体重和TC、TAG、HDL-C、LDL-C水平以及GLUT1、caveolin-1、flotillin-1蛋白表达水平均显著降低，血糖水平和protein4.2蛋白表达水平均显著升高（P＜0.05）；补阳还五汤低、中剂量组小鼠红细胞形态明显改善。结论补阳还五汤可降低高脂血症模型小鼠的血脂水平，改善高脂环境中红细胞的形态及功能，有预防高脂血症并发症动脉粥样硬化的潜力。 |
| ABSTRACT： | OBJECTIVE To investigate the effects of Buyang huanwu decoction on red blood cells in hyperlipidemia model mice. METHODS Male C57BL/6 mice were fed a high-fat diet to prepare a hyperlipidemia model. Successfully modeled mice were randomly assigned to the model group， atorvastatin calcium group （0.26 g/kg）， fenofibrate group （1.3 mg/kg）， and high-， medium-， and low- dose groups of Buyang huanwu decoction （18.6， 9.3， 4.6 g/kg）， with 10 mice in each group. Additionally， 10 mice fed with regular chow served as the normal group. Each group of mice received intragastric administration with the corresponding drug or normal saline once daily for 21 consecutive days. After the last administration， the body weight of the mice in each group was measured， and blood glucose， total cholesterol （TC）， triglyceride （TAG）， high-density lipoprotein cholesterol （HDL-C）， and low-density lipoprotein cholesterol （LDL-C） levels were measured. Red blood cell morphology changes were observed， and the expression levels of glucose transporter type 1 （GLUT1）， erythrocyte membrane protein band 4.2 （protein 4.2）， caveolin-1， and flotillin-1 in erythrocyte membrane were determined. RESULTS Compared with the model group， the body weight and levels of TC， TAG， HDL-C， LDL-C， and the expression levels of GLUT1， caveolin-1 and flotillin-1 in the Buyang huanwu decoction groups were significantly decreased， while blood glucose levels and the expression levels of protein 4.2 were significantly increased （P＜0.05）. The red blood cell morphology in the low- and medium-dose groups of Buyang huanwu decoction was significantly promoted. CONCLUSIONS Buyang huanwu decoction can reduce blood lipid levels in hyperlipidemia model mice and strengthen the morphology and function of red blood cells in a hyperlipidemic environment， which has the potential to be used for preventing hyperlipidemia complications such as atherosclerosis. |
| 期刊： | 2024年第35卷第22期 |
| 作者： | 周云越；杨金彪；陈星彤；杨蕊红；肖洪彬；牛雯颖 |
| AUTHORS： | ZHOU Yunyue，YANG Jinbiao，CHEN Xintong，YANG Ruihong，XIAO Hongbin，NIU Wenying |
| 关键字： | 补阳还五汤；高脂血症；血脂；红细胞；红细胞膜蛋白 |
| KEYWORDS： | Buyang huanwu decoction； hyperlipidemia； blood lipids； red blood cells； erythrocyte membrane proteins |
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