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姜黄素对子宫内膜异位症模型大鼠的影响及机制
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| 篇名： | 姜黄素对子宫内膜异位症模型大鼠的影响及机制 |
| TITLE： | Impact and mechanism of curcumin on endometriosis model rats |
| 摘要： | 目的 基于Notch1信号通路探讨姜黄素对子宫内膜异位症（EMS）模型大鼠的影响及机制。方法在动情周期趋于一致的雌性SD大鼠腹壁上种植自体子宫内膜，构建EMS模型，并将其随机分为模型组，姜黄素低、中、高剂量组（60、120、240mg/kg）和Notch1抑制剂DAPT组（7mg/kg），另设假手术组，每组10只。各组大鼠灌胃或尾静脉注射相应药物，给药4周后，分别通过超声仪和肉眼观察、测量子宫内膜异位病灶并计算体积；观察病灶组织病理形态；检测大鼠腹腔液中白细胞介素1β（IL-1β）、IL-6和肿瘤坏死因子α（TNF-α）含量，以及子宫内膜异位病灶中Notch1、基质金属蛋白酶9（MMP-9）和血管内皮生长因子（VEGF）mRNA和蛋白表达水平。结果与模型组比较，姜黄素低、中、高剂量组和DAPT组大鼠子宫内膜异位病灶的体积均显著减小（P＜0.05），柱状上皮萎缩或消失，囊泡消失；腹腔液中IL-1β、IL-6和TNF-α（姜黄素低剂量组除外）水平以及子宫内膜异位病灶中Notch1、MMP-9和VEGFmRNA（姜黄素低剂量组MMP-9、VEGF除外）及蛋白（姜黄素低剂量组MMP-9除外）表达水平均显著降低（P＜0.05），其中姜黄素高剂量组和DAPT组大部分指标优于姜黄素低、中剂量组。结论姜黄素对EMS具有改善作用，其作用机制可能与抑制Notch1信号通路、减轻局部炎症反应、抑制异位内膜的侵袭和血管生成有关。 |
| ABSTRACT： | OBJECTIVE To explore the impact and mechanism of curcumin on endometriosis （EMS） model rats based on Notch1 signaling pathway. METHODS Female SD rats with synchronized estrous cycles were implanted with autologous endometrium on the abdominal wall to construct EMS model. EMS rats were randomly divided into model group， low-， medium- and high-dose groups of curcumin （60， 120， 240 mg/kg）， and Notch 1 inhibitor DAPT group （7 mg/kg）. The sham surgery group was also established， with 10 rats in each group. Rats in each group received intragastric administration or injection via caudal veins with the corresponding drugs for 4 weeks. Endometriotic lesions were observed and measured using ultrasound and visual inspection， and their volumes were calculated. Histopathological morphology of the lesion tissues was observed. The levels of interleukin-1β （IL-1β）， IL-6， and tumor necrosis factor-α （TNF-α） in the abdominal cavity fluid， as well as the mRNA and protein expression levels of Notch1， matrix metalloproteinase-9 （MMP-9）， and vascular endothelial growth factor （VEGF） in the endometriotic lesions were measured. RESULTS Compared with the model group， the volume of endometriotic lesions in the low- ， medium- and high-dose groups of curcumin and the DAPT group was significantly decreased （P＜0.05）； atrophy or disappearance of columnar epithelium， cyst disappearance； the levels of IL-1β， IL-6， and TNF- α （except for the low-dose curcumin group） in the abdominal cavity fluid， as well as the mRNA （except for MMP-9 and VEGF in the low-dose curcumin group） and protein （except for MMP-9 in the low-dose curcumin group） expression levels of Notch1， MMP-9， and VEGF in the endometriotic lesions were significantly decreased （P＜0.05）. The curcumin high-dose group and DAPT group showed superior results in most indicators compared to the curcumin low- and medium-dose groups. CONCLUSIONS Curcumin has an improving effect on EMS， and its mechanism may be related to inhibiting Notch1 signaling pathway， reducting local inflammatory responses， and inhibiting ectopic endometrial invasion and angiogenesis. |
| 期刊： | 2024年第35卷第22期 |
| 作者： | 秦翠梅；刘心怡；丁晓兰；郭会领；陈品英；刘姣；程杰 |
| AUTHORS： | QIN Cuimei，LIU Xinyi，DING Xiaolan，GUO Huiling，CHEN Pinying，LIU Jiao，CHENG Jie |
| 关键字： | 子宫内膜异位症；姜黄素；Notch1信号通路 |
| KEYWORDS： | endometriosis； curcumin； Notch1 signaling |
| 阅读数： | 6 次 |
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