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化痰通脉饮对多囊卵巢综合征大鼠的改善作用机制研究
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| 篇名： | 化痰通脉饮对多囊卵巢综合征大鼠的改善作用机制研究 |
| TITLE： | Study on the improvement mechanism of Huatan tongmai decoction on rats with polycystic ovary syndrome |
| 摘要： | 目的 基于磷脂酰肌醇3-激酶（PI3K）/蛋白激酶B（AKT）/哺乳动物雷帕霉素靶蛋白（mTOR）通路调控自噬途径，探讨化痰通脉饮对多囊卵巢综合征（PCOS）大鼠的改善作用机制。方法将40只大鼠随机分为空白组（纯净水）、模型组（纯净水）、中药组[化痰通脉饮，24g/（kg·d）]和化学药组[二甲双胍，0.16g/（kg·d）]，每组10只。除空白组外，其余各组均采用灌胃1mg/kg来曲唑混悬液联合高脂饲料喂养的方法建立PCOS大鼠模型。造模成功后，分别灌胃给药或水，每天1次，连续42d。末次给药后，观察大鼠卵巢组织的病理形态学与超微结构变化；检测大鼠血清中促卵泡素（FSH）、睾酮（T）、雌二醇（E2）、促黄体素（LH）水平并计算LH/FSH比值；检测大鼠卵巢组织中苄氯素1（Beclin-1）、p62、微管相关蛋白1轻链3（LC3）的mRNA表达水平以及PI3K/AKT/mTOR通路和自噬相关蛋白表达水平。结果与空白组比较，模型组大鼠卵巢组织病理损伤和超微结构改变明显，可见胞内大量自噬小体；血清中T、LH水平和LH/FSH比值，卵巢组织中Beclin-1、LC3mRNA及其蛋白表达水平均显著升高（P＜0.05）；血清中E2、FSH水平，卵巢组织中p62mRNA及其蛋白表达水平和PI3K、AKT、mTOR蛋白的磷酸化水平均显著降低（P＜0.05）。与模型组比较，给药组大鼠卵巢组织的病理损伤明显减轻，自噬小体数量减少，上述指标的表达水平均被显著逆转（P＜0.05）。结论化痰通脉饮可通过激活PI3K/AKT/mTOR通路，抑制卵巢颗粒细胞自噬，调节性激素分泌，减轻卵巢组织的病理损伤，促进卵泡正常发育，从而发挥对PCOS大鼠的改善作用。 |
| ABSTRACT： | OBJECTIVE To investigate the improvement mechanism of Huatan tongmai decoction on rats with polycystic ovary syndrome （PCOS） by regulating autophagy through phosphatidylinositol-3-kinase（PI3K）/protein kinase B（AKT）/mammalian target of rapamycin （mTOR） pathway. METHODS A total of 40 rats were randomly divided into blank group （purified water）， model group （purified water）， traditional Chinese medicine group [Huatan tongmai decoction， 24 g/（kg·d）] and chemical drug group [metformin， 0.16 g/（kg·d）]， with 10 rats in each group. Except for blank group， other groups were given a combination of high-fat diet and intragastric administration of 1 mg/kg letrozole suspension to establish PCOS rat model. After modeling， they were given relevant medicine or water intragastrically， once a day， for 42 consecutive days. After the last administration， the pathological and ultrastructural changes of ovarian tissue were observed. The levels of follicle stimulating hormone （FSH）， testosterone （T）， estradiol （E2） ，luteinizing hormone （LH） in serum were detected，and the LH/FSH ratio was calculated. mRNA expressions of Beclin-1， p62 and microtubule-associated protein 1 light chain 3 （LC3） in ovarian tissue were detected. The expressions of related proteins of PI3K/AKT/mTOR pathway and autophagy in rat ovarian tissues were also detected. RESULTS Compared with blank group， the pathological damage and ultrastructural changes of the ovarian tissue in the model group rats were obvious， and a large number of autophagosomes could be seen in cells. The levels of T and LH and the LH/FSH ratio in serum， as well as mRNA and protein expressions of Beclin-1 and LC3， were increased significantly （P＜0.05）， while the levels of E2 and FSH in serum， as well as mRNA and protein expressions of p62 and the phosphorylation levels of PI3K， AKT and mTOR proteins in ovarian tissue， were significantly decreased （P＜0.05）. Compared with model group， the pathological damage of ovarian tissue in the administration groups was significantly reduced， the number of autophagosomes was smaller， and the expression levels of the above indicators were significantly reversed （P＜0.05）. CONCLUSIONS Huatan tongmai decoction can inhibit autophagy in ovarian granular cells by activating the PI3K/AKT/mTOR pathway， regulate the secretion of sex hormones， alleviate pathological damage in ovarian tissues， and promote normal follicular development， thereby exerting an ameliorative effect on PCOS rats. |
| 期刊： | 2025年第36卷第23期 |
| 作者： | 姚新亚；林寒梅；逯克娜；甘子晴；郭华；李文意；李健玲；金奇霖 |
| AUTHORS： | YAO Xinya，LIN Hanmei，LU Kena，GAN Ziqing，GUO Hua，LI Wenyi，LI Jianling，JIN Qilin |
| 关键字： | 化痰通脉饮；多囊卵巢综合征；PI3K/AKT/mTOR通路；自噬；颗粒细胞 |
| KEYWORDS： | Huatan tongmai decoction； polycystic ovary syndrome； PI3K/AKT/mTOR pathway； autophagy； granular cells |
| 阅读数： | 1 次 |
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