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复方蜥蜴散诱导细胞凋亡及自噬性死亡逆转胃癌顺铂耐药的机制研究
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| 篇名： | 复方蜥蜴散诱导细胞凋亡及自噬性死亡逆转胃癌顺铂耐药的机制研究 |
| TITLE： | Study on the mechanism of Compound lizard powder on reversing cisplatin resistance in gastric cancer by inducing apoptosis and autophagic death |
| 摘要： | 目的 研究复方蜥蜴散通过核因子κB（NF-κB）/SNAIL信号通路逆转顺铂（DDP）耐药性胃癌细胞MKN45/DDP的耐药机制。方法将MKN45/DDP细胞分为模型组（20%正常大鼠血清）、DDP组（1.3μg/mLDDP+20%胎牛血清）及复方蜥蜴散低、高剂量含药血清+DDP组（17.2、68.8g/kg复方蜥蜴散的20%含药血清+1.3μg/mLDDP）。测定MKN45/DDP细胞的生存活力、凋亡水平、自噬水平，检测细胞上清中微管相关蛋白1轻链3（LC3）含量，检测细胞中B细胞淋巴瘤2（Bcl-2）、Bcl-2相关X蛋白（Bax）表达水平以及磷酸化NF-κBp65（p-NF-κBp65）、SNAIL的蛋白表达水平；利用NF-κB通路激动剂肿瘤坏死因子α（TNF-α）进一步明确复方蜥蜴散改善DDP耐药性的分子机制。结果与模型组及DDP组比较，复方蜥蜴散低、高剂量含药血清+DDP组细胞存活率均显著降低（P＜0.05），凋亡及自噬水平均显著升高（P＜0.05）；细胞中Bcl-2（复方蜥蜴散低剂量含药血清+DDP组除外）、p-NF-κBp65、SNAIL蛋白表达水平均显著降低（P＜0.05），LC3含量和Bax蛋白表达水平均显著升高（P＜0.05）。复方蜥蜴散高剂量含药血清+DDP可有效逆转TNF-α诱导的耐药细胞中LC3Ⅱ/LC3Ⅰ比值下降及Bax蛋白表达下调（P＜0.05）。结论复方蜥蜴散含药血清联合DDP可通过抑制NF-κB/SNAIL信号通路，诱导MKN45/DDP细胞凋亡及自噬性死亡，从而逆转该细胞的DDP耐药性。 |
| ABSTRACT： | OBJECTIVE To study the mechanism of Compound lizard powder on reversing cisplatin（DDP） resistance in resistant gastric cancer cell MKN45/DDP through nuclear factor-κB （NF-κB）/SNAIL signaling pathway. METHODS MKN45/DDP cells were divided into model group （20% normal rat serum）， DDP group （1.3 μg/mL DDP+20% fetal bovine serum） and Compound lizard powder low- and high-dose drug-containing serum+DDP groups （17.2， 68.8 g/kg Compound lizard powder 20% drug-containing serum+1.3 μg/mL DDP）. The viability， apoptosis and intracellular autophagosome of MKN45/DDP cells were detected. The content of microtubule-associated protein 1 light chain 3 （LC3） in the cell supernatant was detected. The expressions of B-cell lymphoma 2 （Bcl-2）， Bcl-2-related X protein （Bax）， as well as the protein expression levels of phosphorylated NF-κB p65 （p-NF-κB p65） and SNAIL were detected. The molecular mechanism of Compound lizard powder in improving DDP resistance was further clarified by using NF-κB pathway agonist tumor necrosis factor-α （TNF-α）. RESULTS Compared with model group and DDP group， the cell survival rates of Compound lizard powder low- and high-dose drug-containing serum+DDP groups were significantly decreased （P＜0.05）， while the levels of apoptosis and autophagic death were significantly increased （P＜0.05）. The expression levels of Bcl-2 （except for the compound lizard powder low-dose drug-containing serum+DDP group）， p-NF-κB p65 and SNAIL protein in MKN45/DDP cells were significantly decreased （P＜0.05）. The content of LC3 and expression of Bax protein were significantly increased （P＜0.05）. High-dose Compound lizard powder drug-containing serum+DDP could effectively reverse the down-regulation of LC3 Ⅱ/LC3 Ⅰ and Bax protein expression induced by TNF- α （P＜0.05）. CONCLUSIONS Compound lizard powder drug-containing serum combined with DDP can induce apoptosis and autophagic death of MKN45/DDP cells by inhibiting NF-κB/SNAIL signaling pathway， thus reversing DDP resistance of cells. |
| 期刊： | 2025年第36卷第23期 |
| 作者： | 刘彩月；李铮；李卫强 |
| AUTHORS： | LIU Caiyue，LI Zheng，LI Weiqiang |
| 关键字： | 复方蜥蜴散；MKN45/DDP细胞；顺铂；耐药性；自噬；凋亡；NF-κB/SNAIL信号通路 |
| KEYWORDS： | Compound lizard powder； MKN45/DDP |
| 阅读数： | 1 次 |
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