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奶制法对蒙药商陆中商陆皂苷甲含量及肝毒性的影响
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| 篇名： | 奶制法对蒙药商陆中商陆皂苷甲含量及肝毒性的影响 |
| TITLE： | Effects of milk processing method on the content of esculentoside A and hepatotoxicity in Mongolian medicine Phytolacca acinosa |
| 摘要： | 目的 研究奶制法对蒙药商陆中商陆皂苷甲含量及肝毒性的影响。方法采用高效液相色谱-蒸发光散射检测法检测生商陆、奶浸泡商陆、奶煮商陆中商陆皂苷甲的含量。将大鼠随机分为正常组、生商陆组、奶浸泡商陆组、奶煮商陆组，每组10只。除正常组外，其余各组大鼠均按0.8g/kg灌胃相应药液，每天1次，连续15d。末次给药后，检测大鼠血浆中生化指标[丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）]和炎症因子[白细胞介素2（IL-2）、IL-6、肿瘤坏死因子α（TNF-α）]水平以及肝组织中丙二醛（MDA）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）水平；观察大鼠肝组织病理学形态变化。结果生商陆、奶浸泡商陆、奶煮商陆中商陆皂苷甲的含量分别为6.46～6.59、4.79～4.89、5.04～5.14mg/g。与正常组比较，生商陆组大鼠血浆中ALT、AST、IL-2、TNF-α水平均显著升高（P＜0.05），肝组织中SOD水平显著降低（P＜0.05）；肝小叶内出现点状坏死灶，肝细胞略有空泡变性表现。与生商陆组比较，奶浸泡商陆组、奶煮商陆组大鼠血浆中ALT、AST水平和肝组织中MDA水平均显著降低（P＜0.05），肝组织中SOD水平均显著升高（P＜0.05）；奶浸泡商陆组大鼠血浆中TNF-α水平和奶煮商陆组大鼠血浆中IL-2、IL-6水平均显著降低（P＜0.05）；奶浸泡商陆组、奶煮商陆组大鼠的肝损伤程度均明显减轻。结论蒙药商陆采用奶制法炮制后的商陆皂苷甲含量和肝毒性均降低。 |
| ABSTRACT： | OBJECTIVE To study the effects of milk processing method on the content of esculentoside A and hepatotoxicity in Mongolian medicine Phytolacca acinosa. METHODS High performance liquid chromatography-evaporative light-scattering detection was used to determine the content of esculentoside A in raw P. acinosa， milk-soaked P. acinosa and milk-boiled P. acinosa. The rats were randomly divided into normal group， raw P. acinosa group， milk-soaked P. acinosa group and milk-boiled P. acinosa group， with 10 rats in each group. Except for the normal group， the rats in the remaining groups were administered corresponding medicinal solutions at a dose of 0.8 g/kg once daily for 15 consecutive days. After the last administration， the levels of biochemical markers [alanine transaminase （ALT）， aspartate transaminase （AST）] and inflammatory factors [interleukin-2 （IL- 2）， IL-6， tumor necrosis factor- α （TNF- α）] in plasma of rats， as well as the levels of malondialdehyde （MDA）， superoxide dismutase （SOD） and glutathione peroxidase （GSH-Px） in liver tissue， were determined. The pathological changes of liver tissue in rats were observed. RESULTS The contents of esculentoside A in raw P. acinosa， milk-soaked P. acinosa and milk-boiled P. acinosa were 6.46-6.59， 4.79-4.89， 5.04-5.14 mg/g， respectively. Compared with the normal group， the plasma levels of ALT， AST， IL-2 and TNF-α in rats were increased significantly in the raw P. acinosa group （P＜0.05）， while the level of SOD in liver tissue was decreased significantly （P＜0.05）； scattered punctate necrotic foci were observed within the hepatic lobules， and hepatocytes exhibited slight vacuolar degeneration. Compared with the raw P. acinosa group， the levels of ALT and AST in plasma of rats and the level of MDA in liver tissue were all decreased significantly in the milk-soaked P. acinosa group and the milk-boiled P. acinosa group （P＜0.05）， while the level of SOD in liver tissue was increased significantly （P＜0.05）. The plasma level of TNF-α in the rats of the milk-soaked P. acinosa group and the plasma levels of IL-2 and IL-6 in the rats of the milk-boiled P. acinosa group were all decreased significantly （P＜0.05）； additionally， the degree of liver injury was markedly alleviated in both groups. CONCLUSIONS The content of esculentoside A and hepatotoxicity both decrease after processing Mongolian medicine P. acinosa using the milk processing method. |
| 期刊： | 2025年第36卷第23期 |
| 作者： | 陈金花；陈红梅；新图雅；周兴安；吴杰斯；包明兰 |
| AUTHORS： | CHEN Jinhua，CHEN Hongmei，Xintuya，ZHOU Xing’an，WU Jiesi，BAO Minglan |
| 关键字： | 蒙药；商陆；炮制；奶制法；商陆皂苷甲；肝毒性 |
| KEYWORDS： | Mongolian medicine； Phytolacca acinosa； processing； milk processing method； esculentoside A； hepatotoxicity |
| 阅读数： | 1 次 |
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