离子色谱法测定骨瓜提取物注射液中糖的含量
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篇名: 离子色谱法测定骨瓜提取物注射液中糖的含量
TITLE:
摘要:

目的:建立测定骨瓜提取物注射液中糖含量的方法。方法:采用离子色谱法。色谱柱为Dionex CarboPac PA20,流动相

为水-1 mol/L 醋酸钠溶液-250 mmol/L氢氧化钠溶液(梯度洗脱),流速为0.45 ml/min,柱温为35 ℃,进样量为10 μl,检测器为电化

学分析检测器。结果:半乳糖、葡萄糖、甘露糖、蔗糖、果糖检测质量浓度线性范围均为0.5~10.0 μg/ml(r>0.998 0);精密度、稳定

性、重复性试验的RSD<2%;各组分加样回收率为94.8%~99.1%,RSD=0.52%~0.89%(n=9)。结论:该方法简单、快速、准

确,可用于骨瓜提取物注射液中糖的测定。

ABSTRACT:

OBJECTIVE:To establish a method for the content determination of carbohydrates in Osseous and cucumis extract

injection. METHODS:Ion chromatography was conducted on the column of Dionex CarboPac PA20 with mobile phase of water-1

mol/L Sodium acetate solution-250 mmol/L Sodium hydroxide solution(gradient elution)at flow rate of 0.45 ml/min,column temperature

was 35 ℃,and injection volume was 10 μl,detector was electrochemical analysis detector. RESULTS:The linear ranges

of galactose,glucose,mannose,sucrose and fructose were 0.5-10.0 μg/ml(r>0.998 0);RSDs of precision,stability and reproducibility

tests were lower than 2%;recovery was 94.8%-99.1%(RSD=0.52%-0.89%,n=9). CONCLUSIONS:The method is simple,

fast and accurate,and can be used for the determination of carbohydrates in Osseous and cucumis extract injection.

期刊: 2016年第27卷第3期
作者: 张伟,张彤,任连杰,吕雯,韩春霞,周长明
AUTHORS: ZHANG Wei,ZHANG Tong,REN Lianjie,LYU Wen,HAN Chunxia,ZHOU Changming
关键字: 离子色谱法;骨瓜提取物注射液;糖
KEYWORDS: Ion chromatography;Osseous and cucumis extraction injection;Carbohydrates
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