目的：建立同时测定安脑丸中栀子苷、盐酸小檗碱和黄芩苷含量的方法。方法：采用高效液相色谱法。色谱柱为Inertsil

ODS-3 C18，流动相为乙腈-0.2％磷酸溶液（梯度洗脱），流速为1.0 ml/min，柱温为30 ℃，检测波长为230 nm，进样量为10 μl。结果：栀子

苷、盐酸小檗碱和黄芩苷的检测进样量线性范围分别为0. 041 0～0.820 0、0. 037 6～0.752 0、0. 095 2～1.904 0 μg（r＝0.999 9）；加样回

收率分别为97.31％～100.41％、98.32％～101.76％、99.16％～101.30％，RSD分别为1.10％、1.20％、0.76％（n＝6）。结论：该方法

简便、准确，可用于安脑丸的质量控制。

OBJECTIVE：To establish a method for simultaneous determination of geniposide，berberine hydrochloride and baicalin

in Annao pill. METHODS：HPLC was performed on the column of Inertsil ODS-3 C18 with mobile phase of acetonitrile -

0.2％ phosphoric acid solution（gradient elution）at a flow rate of 1.0 ml/min，the column temperature was 30 ℃，the detection

wavelength was 230 nm，and the injection volume was 10 μl. RESULTS：The linear range was 0. 041 0-0.820 0 μg for geniposide

（r＝0.999 9），0.037 6-0.752 0 μg for berberine hydrochloride（r＝0.999 9）and 0.095 2-1.904 0 μg for baicalin（r＝0.999 9），respectively；

recoveries were 97.31％ -100.41％（RSD＝1.10％ ，n＝6），98.32％ -101.76％（RSD＝1.20％ ，n＝6） and 99.16％ -101.30％

（RSD＝0.76％，n＝6），respectively. CONCLUSIONS：The method is simple and accurate，and can be used for the quality control

of Annao pill.