目的：建立柴葛感冒合剂的质量标准。方法：采用薄层色谱（TLC）法对制剂中黄芩、白芍进行定性鉴别。采用高效液相

色谱法测定制剂中葛根素的含量：色谱柱为Zorbax Eclipse XDB-C18，流动相为甲醇-水（25 ∶75，V/V），流速为1.0 ml/min，检测波长

为250 nm，柱温为25 ℃，进样量为5 μl。结果：TLC图斑点清晰，分离度好。葛根素检测质量浓度线性范围为18～198 μg/ml（r＝

0.999 9）；精密度、稳定性、重复性试验的RSD＜2％；加样回收率为99.04％～99.75％，RSD＝0.28％（n＝6）。结论：所建标准可用

于柴葛感冒合剂的质量控制。

OBJECTIVE：To establish the quality standard for Chaige ganmao mixture. METHODS：TLC was conducted to identify

the Scutellaria baicalensis and Paeonia lactiflora；HPLC was conducted to determine the content of puerarin. The column was

Zorbax Eclipse XDB-C18 with mobile phase of methanol-water（25 ∶ 75，V/V）at flow rate of 1.0 ml/min，detection wavelength was

250 nm，and column temperature was 25 ℃，and the injection volume was 5 μl. RESULTS：TLC showed clear spots and good separation.

The linear range of puerarin was 18-198 μg/ml（r＝0.999 9）；RSDs of precision，stability and reproducibility tests were lower

than 2％，recovery was 99.04％-99.75％（RSD＝0.28％，n＝6）. CONCLUSIONS：The standard can be used for the quality control

of Chaige ganmao mixture.