目的：建立两头尖的高效液相色谱（HPLC）指纹图谱。方法：采用HPLC法。色谱柱为Phemomenex Gemini C18，流动相为

0.1％磷酸-乙腈（梯度洗脱），流速为1 ml/min，检测波长为206 nm，柱温为30 ℃，进样量为20 μl。以竹节香附素A为参照物，分析

13 批两头尖样品，采用“中药色谱指纹图谱相似度评价系统”软件进行相似度分析，并采用SPSS 13.0 统计软件对样品进行聚类分

析。结果：13 批两头尖样品有11 个共有峰，相似度＞0.90。经验证，两头尖的指纹图谱与对照指纹图谱具有较好的一致性。13 批

药材产地中吉林桦甸、吉林蛟河、吉林天岗、吉林左家、黑龙江尚志、吉林舒兰、吉林通化、吉林抚松为第1 类，黑龙江哈尔滨、黑龙

江亚布力镇、辽宁清原、黑龙江一面坡、山东济南为第2类。结论：所建指纹图谱可为两头尖的鉴别和质量评价提供参考。

OBJECTIVE：To establish the HPLC fingerprint for Anemone raddeana. METHODS：HPLC was performed on the

column of Phemomenex Gemini C18 with mobile phase of 0.1％ phosphoric acid-acetonitrile（gradient elution）at a flow rate of 1 ml/

min，the detection wavelength was 206 nm，the column temperature was 30 ℃，and the injection volume was 20 μl. With the reference

of raddeanin A，13 batches of A. raddeana were analyzed，chromatographic fingerprint similarity evaluation system software

was conducted for similarity analysis，and SPSS 13.0 was conducted for cluster analysis. RESULTS：There were 11 common peaks

in the 13 batches of A. raddeana with similarity of higher than 0.90. According to the verification，the fingerprint and control fingerprint

shows good consistency. The drugs in Huadian，Jiaohe， Tiangang，Shulan，Tonghua and Fusong of Jilin and Shangzhi of

Heilongjiang were regarded as category 1，and in Harbin，Yabuli town and Yimianpo of Heilongjiang，Qingyuan of Liaoning，Jinan

of Shandong were category 2. CONCLUSIONS：The established fingerprint can provide reference for the identification and

quality evaluation of A. raddeana.