目的：建立测定脑血栓片中胆酸、猪去氧胆酸和去氧胆酸含量的方法。方法：采用高效液相色谱-蒸发光散射检测法。色

谱柱为SHIMADZU VP-ODS C18，检测器为蒸发光散射检测器，流动相为乙腈-0.2％甲酸水溶液（梯度洗脱），流速为1.0 ml/min，柱

温为30 ℃，进样量为10 μl。结果：胆酸、猪去氧胆酸和去氧胆酸进样量分别在0.039 84～0.398 4、0.032 064～0.320 64、0.033 952～

0.339 52 mg（r≥0.999 0）范围内进样量的对数值与其峰面积的对数值呈良好的线性关系；精密度、稳定性、重复性试验的RSD＜

2％；加样回收率分别为101.33％～104.05％、95.56％～101.38％、96.07％～97.12％，RSD分别为0.95％、2.54％、0.44％（n＝6）。结

论：该方法简便、准确，适用于脑血栓片中胆酸、猪去氧胆酸和去氧胆酸的含量测定。

OBJECTIVE：To establish a method for the contents determination of cholic acid，hyodeoxycholic acid and deoxycholate

acid in Naoxueshuan tablet. METHODS：HPLC-ELSD was performed on the column of SHIMADZU VP-ODS C18 with mobile

phase of acetonitrile-0.2％ formic acid（gradient elution）column temperature was 30 ℃，at flow rate of 1.0 ml/min，and the volume

injection was 10 μl. RESULTS：The linear range was 0.039 84-0.398 4 mg for cholic acid，0.032 064-0.320 64 mg for hyodeoxycholic

acid and 0.0339 52-0.339 52 μg for deoxycholate acid（r≥0.999 0），and the logarithm value of volume injection and peak

area showed good linear relationship；RSDs of precision，stability and reproducibility tests were lower than 2％；recoveries were

101.33％-104.05％（RSD＝0.95％，n＝6），95.56％-101.38％（RSD＝2.54％，n＝6）and 96.07％-97.12％（RSD＝0.44％，n＝6），respectively.

CONCLUSIONS：The method is simple and accurate，and suitable for the contents determination of cholic acid，hyodeoxycholic

acid and deoxycholate acid in Naoxueshuan tablet.