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正交试验法优化微波协同双水相提取甘草中总黄酮的工艺
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| 篇名： | 正交试验法优化微波协同双水相提取甘草中总黄酮的工艺 |
| TITLE： | |
| 摘要： | 目的：优化微波协同双水相提取甘草中总黄酮的工艺。方法： 以硫酸铵和乙醇为双水相萃取体系，微波法提取甘草中的总黄酮；采用单因素试验和正交试验考察硫酸铵质量浓度、乙醇体积分数、固液比、微波功率、提取时间 5个因素对总黄酮提取率的影响，优化提取工艺并进行验证试验。结果：最优提取工艺为硫酸铵质量浓度0.40 g/ml、乙醇体积分数70％、固液比1 ∶ 25、提取时间3 min、微波功率300 W；验证试验中甘草总黄酮平均提取率为2.038％（RSD＝0.69％，n＝3），双水相萃取率为94.46％（RSD＝1.30％，n＝3）。结论：该工艺操作简单、结果稳定、反应条件温和、提取效率较高，适合工业化提取甘草中总黄酮。 |
| ABSTRACT： | OBJECTIVE： To optimize microwave-assisted aqueous two-phase system of total flavonoids from Glycyrrhiza uralensis. METHODS： The ammonium sulfate and ethanol were used as aqueous two-phase extraction system， the method of microwave was applied to extract total flavonoids from G. uralensis. The effects of 5 factors on extraction rate of total flavonoids were investigated by single factor test and orthogonal test， such as the mass concentration of ammonium sulfate， ethanol volume fraction， solid-liquid ratio， microwave power and extraction time. Validation test was conducted. RESULTS： The optimal extraction condition was as follows as the mass concentration of ammonium sulfate of 0.40 g/ml， ethanol volume fraction of 70％， solid-liquid ratio of 1 ∶ 25， extraction time of 3 min and microwave power of 300 W； in validation test， the extraction rate of total flavonoids from G. uralensis was 2.038％ （RSD＝0.69％， n＝3）， and the extraction rate of two-phase system was 94.46％ （RSD＝1.30％， n＝3）. CONCLUSIONS： The technology is simple in operation and stable in results， and also has gentle reaction conditions and high extraction rate， which is suitable for industrial production. |
| 期刊： | 2016年第27卷第4期 |
| 作者： | 陈丽娟，柳福智，岳强兵 |
| AUTHORS： | CHEN Lijuan，LIU Fuzhi，YUE Qiangbing |
| 关键字： | 正交试验；微波；双水相；甘草；总黄酮 |
| KEYWORDS： | Orthogonal test； Microwave； Aqueous two-phase； Glycyrrhiza uralensis； Total flavonoids |
| 阅读数： | 262 次 |
| 本月下载数： | 5 次 |
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