公石松抗运动性疲劳的活性部位筛选
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篇名: 公石松抗运动性疲劳的活性部位筛选
TITLE:
摘要: 目的:筛选公石松抗运动性疲劳的活性部位。方法:用80%乙醇提取制备公石松浸膏,用水分散后依次用石油醚、氯仿、乙酸乙酯、正丁醇进行萃取,得各部位提取物。将70只小鼠随机分为空白对照(1%羧甲基纤维素钠,CMC-Na)组、阳性对照[大株红景天胶囊,590 mg/(kg·d)]组和公石松石油醚、氯仿、乙酸乙酯、正丁醇提取物组及水层组(分别记为TS、TL、TY、TZ、TW组),公石松提取物组小鼠均按2.5 g(生药)/(kg·d) ig给药,连续7 d,测定小鼠负重游泳力竭时间。将70只小鼠按上述方法分组给药,测定小鼠体内肝糖原、肌糖原含量和肝脏系数。将80只小鼠除按上述方法分组给药外增设模型(1% CMC-Na溶液)组,不负重游泳90 min后测定小鼠血清中乳酸、肌酸激酶及尿素氮含量。结果:与空白对照组比较,TS、TY、TZ组小鼠负重游泳力竭时间延长,TY、TZ、TW组小鼠体内肝糖原含量增加,TS、TW组小鼠体内肌糖原含量增加,模型组小鼠血清中尿素氮、乳酸、肌酸激酶含量增加(P<0.05或P<0.01);与模型组比较,TS、TY组小鼠血清中尿素氮含量减少,TZ组及TW组小鼠血清中乳酸含量减少,TS组小鼠血清中肌酸激酶含量减少(P<0.05或P<0.01)。结论:公石松的石油醚、正丁醇部位和水层具有较好的抗疲劳活性。
ABSTRACT: OBJECTIVE: To screen Gongshisong’s active sites for anti-sports fatigue. METHODS: Gongshisong extract was prepared with 80% ethanol extraction technology, and extracted with petroleum ether, chloroform, ethyl acetate and n-butyl alcohol after dispersed with water to obtain the extract. 70 mice were randomly divided into blank control group (1% sodium carboxymethylcellulose, CMC-Na), positive control group [Rhodiola wallichiana capsules, 590 mg/(kg·d)], petroleum ether, chloroform, ethyl acetate and n-butyl alcohol extracts and aqueous layer of Gongshisong groups (TS, TL, TY, TZ, TW groups). Gongshisong extracts groups was given relevant medicine 2.5 g(crude drug)/(kg·d), ig, for consecutive 7 days. Exhaustion time of burden swimming test was detected. 70 mice were grouped according to above method, and the contents of liver glycogen, muscle glycogen and the coefficient of liver were tested in mice. 80 mice were grouped according to above method, and model group was established additionally (1% CMC-Na). The contents of lactic acid (LA), creatine kinase (CK) and urea nitrogen (BUN) in serum of mice were determined after 90 minutes of unburden swimming. RESULTS: Compared with blank control group, exhaustion time of burden swimming mice in TS, TY and TZ groups prolonged; the content of liver glycogen increased in TY, TZ and TW groups; the content of muscle glycogen increased in TS and TW groups; the contents of BUN, LA and CK in mice increased in model group (P<0.05 or P<0.01). Compared with model group, the serum content of BUN in mice decreased in TS and TY groups; that of LA in mice decreased in TZ and TW groups; that of CK in mice decreased in TS group (P<0.05 or P<0.01). CONCLUSIONS: The petroleum ether and n-butanol extract site and water layer of Gongshisong are good anti-fatigue active sites.
期刊: 2016年第27卷第10期
作者: 石鹤坤,邱韵玲,陈开杰,杨钦磊,陈锦珊
AUTHORS: SHI Hekun,QIU Yunling,CHEN Kaijie,YANG Qinlei,CHEN Jinshan
关键字: 公石松;活性部位;抗疲劳;负重游泳;糖原;小鼠
KEYWORDS: Gongshisong; Active site; Anti-fatigue; Burden swimming; Glycogen; Mice
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