虎石灌肠颗粒的体外抑菌作用考察
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篇名: 虎石灌肠颗粒的体外抑菌作用考察
TITLE:
摘要: 目的:考察虎石灌肠颗粒的体外抑菌作用。方法:采用试管二倍稀释法测定虎石灌肠颗粒药液[初始质量浓度为1 200 mg (生药)/ml]对金黄色葡萄球菌、大肠埃希菌、沙门菌、志贺氏痢疾杆菌、福氏痢疾杆菌的最低抑菌浓度(MIC);采用琼脂培养基平板法测定其最低杀菌浓度(MBC);选择4 MIC和2 MIC药液测定杀菌速度(菌液浓度均为1.5×105 CFU/ml),并绘制杀菌曲线(KCS)图。结果:虎石灌肠颗粒药液对金黄色葡萄球菌、大肠埃希菌、沙门菌、志贺氏痢疾杆菌、福氏痢疾杆菌的MIC分别为18.75、150、150、75、150 mg(生药)/ml,MBC分别为37.50、150、150、75、150 mg(生药)/ml;KCS图显示,4MIC、2MIC药液对5种受试菌株均有较好的杀灭作用,全部杀灭所需时间分别为24、1、1、7、1 h和24、7、3、7、7 h。结论:虎石灌肠颗粒具有较强的体外抑菌作用。
ABSTRACT: OBJECTIVE: To study antibacterial effect of Hushi enema granules in vitro. METHODS: Minimal inhibitory concentration (MIC) of Hushi enema granules [initial concentration of 1 200 mg (crude drug)/ml] to Staphylococcus aureus, Escherichia coli, Salmonella, Shigella dysenteriae and Shigella flexneri were detected by tube double dilution method. The minimal bactericidal concentration (MBC) were determined by agar medium plate method; 4 MIC and 2 MIC Hushi enema granules solution were selected to determine sterilizing speed (bacterium concentration of 1.5×105 CFU/ml) and draw time-kill curves (KCS).  RESULTS: MIC of Hushi enema granules to S. aureus, E. coli, Salmonella, S. dysenteriae and S. flexneri were 18.75, 150, 150, 75 and 150 mg(crude drug)/ml, respectively; MBC of Hushi enema granules were 37.50, 150, 150, 75 and 150 mg(crude drug)/ml, respectively. Both 4 MIC and 2 MIC solution had good sterilizing effects on 5 bacterial strains. KCs of 4 MIC and 2 MIC solution sterilizing 5 bacterial strains were 24, 1, 1, 7, 1 h and 24, 7, 3, 7, 7 h, respectively.  CONCLUSIONS: Hushi enema granules show a strong antibacterial effect in vitro.
期刊: 2016年第27卷第13期
作者: 吴嵩,廖佑荣,谭璐,张广求
AUTHORS: WU Song,LIAO Yourong,TAN Lu,ZHANG Guangqiu
关键字: 虎石灌肠颗粒;体外抑菌作用;最低抑菌浓度;最低杀菌浓度;杀菌曲线
KEYWORDS: Hushi enema granules; Antibacterial effect in vitro; Minimal inhibitory concentration; Minimal bactericidal concentration; Time-kill curves
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