HPLC法测定山柰药材中山柰素的含量
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篇名: | HPLC法测定山柰药材中山柰素的含量 |
TITLE: | |
摘要: | 目的:建立测定山柰药材中山柰素含量的方法。方法:采用高效液相色谱法。色谱柱为 Diamonsil ODS2 C18,流动相为甲醇-0.4%磷酸溶液,流速为1 ml/min,检测波长为367 nm,柱温为30 ℃,进样量为10 μl。结果:山柰素的检测质量浓度线性范围为0.001 58~0.158 mg/ml;精密度、稳定性、重复性试验的RSD<3%;加样回收率为95.52%~99.32%,RSD=1.47%(n=6)。结论:该方法操作简单、结果准确、重复性较好,可用于山柰药材中山柰素的含量测定。 |
ABSTRACT: | OBJECTIVE: To establish a method for the content determination of kaempferol in Kaempferia galanga. METHODS: HPLC was performed on the column of Diamonsil ODS2 C18 with mobile phase of methanol-0.4% Phosphoric acid solution at a flow rate of 1 ml/min, detection wavelength was 367 nm, column temperature was 30℃, and injection volume was 10 μl. RESULTS: The linear range of kaempferol was 0.001 58-0.158 mg/ml; RSDs of precision, stability and reproducibility tests were lower than 3%; recovery was 95.52%-99.32% (RSD=1.47%,n=6). CONCLUSIONS: The method is simple, accurate and reproducible, and can be used for the content determination of kaempferol in K. galanga. |
期刊: | 2016年第27卷第18期 |
作者: | 李刚,封传华,张静,徐兰,陶晓璇 |
AUTHORS: | LI Gang,FENG Chuanhua,ZHANG Jing,XU Lan,TAO Xiaoxuan |
关键字: | 高效液相色谱法;山柰;山柰素 |
KEYWORDS: | HPLC; Kaempferia galanga; Kaempferol |
阅读数: | 400 次 |
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