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甘草对大鼠体内雷公藤甲素和雷公藤内酯酮代谢物水平的影响
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| 篇名： | 甘草对大鼠体内雷公藤甲素和雷公藤内酯酮代谢物水平的影响 |
| TITLE： | |
| 摘要： | 目的：研究甘草对大鼠体内雷公藤甲素（TPL）和雷公藤内酯酮（TPN）内源代谢物水平的影响。方法：取160只大鼠ig甘草[30 g（生药）/kg]2 h后ip TPN（2.8 mg/kg），连续30 d，收集24 h尿液，合并30 d尿液，采用柱色谱技术和波谱技术分离鉴定其内源代谢物；并采用超高效液相色谱-高分辨飞行时间质谱联用法（UPLC-Q-TOF/MS）确定TPL给药后是否具有共同代谢物。将60只大鼠随机分为TPL空白组、TPL组、甘草+TPL组、TPN空白组、TPN组、甘草+TPN组，各给药组大鼠给药方式及剂量同上，TPL空白组和TPN空白组大鼠分别ip相应溶剂，每天1次，连续7 d；采用UPLC-Q-TOF/MS法研究甘草对大鼠体内TPL、TPN内源代谢物水平的影响。结果：从TPN给药大鼠尿液中共分离出5个内源代谢物，分别鉴定为1-甲基海因、4-氨基烟酸甲酯、3， 4-二氢-6-羟基-2-喹啉酮、2，6-二羟基喹啉、2-喹啉酮；TPL给药后大鼠尿液中同样存在这5种代谢物。TPL组大鼠尿液中1-甲基海因水平较TPL空白组显著升高（P＜0.05），TPN组大鼠尿液中2，6-二羟基喹啉水平较TPN空白组显著升高（P＜0.05）；TPL或TPN联合甘草用药后血清中1-甲基海因、2，6-二羟基喹啉水平均降低，较其空白组差异无统计学意义（P＞0.05）。结论：本实验首次从TPL和TPN给药大鼠尿液中分离鉴定出了5个内源代谢物；甘草能回调TPL给药大鼠尿液中1-甲基海因水平以及TPN给药后大鼠尿液中2，6-二羟基喹啉水平。 |
| ABSTRACT： | OBJECTIVE： To study the effects of Glycyrrhiza uralensis on endogenous metabolites levels of triptolide （TPL） and triptonide （TPN） in rats. METHODS： 160 rats were given G. uralensis [30 g （crude drug）/kg] intragastrically， 2 h later given TPN （2.8 mg/kg） intraperitoneally for consecutive 30 d. 24 h urine were collected for 30 days， and then endogenous metabolites were separated and identified by column chromatograph and spectrum technology. UPLC-Q-TOF/MS was used to determine the common metabolites after giving TPL. 60 rats were randomly divided into TPL blank group， TPL group， G. uralensis+TPL group， TPN blank group， TPN group， G. uralensis+TPN group. Route of administration and dosage of treatment groups were same as above groups. TPL blank group and TPN blank group were given corresponding solvent， once a day， for consecutive 7 d. UPLC-Q-TOF/MS was used to determine the endogenous metabolites of TPL and TPN in rats. RESULTS： Five endogenous metabolites were isolated and identified from urine of rats after given TPN， as 1-methylhydantoin， methyl 4-aminonicotinate， 3，4-dihydro-6-hydroxy-2（1H）-quinolinone， 2，6-quinolinediol， 2-quinolone， respectively. Those five metabolites were also found in urine of rats after giving TPN. 1-methylhydantoin in TPL group and 2，6-quinolinediol in TPN group were significantly increased， compared with TPL blank group or TPN blank group （P＜0.05）； while urine levels of 1-methylhydantoin and 2，6-quinolinediol decreased after TPL or TPN combining with G. uralensis， compared with TPL/TPN blank group， with no significant difference （P＞0.05）. CONCLUSIONS： The above five endogenous metabolites were isolated from rat urine after given TPL or TPN for the first time. The levels of 1-methylhydantoin after giving TPL and 2，6-quinolinediol in urine after giving TPN can be recovered by G. uralensis. |
| 期刊： | 2016年第27卷第28期 |
| 作者： | 刘建群，张国华，余昭芬，王雪梅 |
| AUTHORS： | LIU Jianqun，ZHANG Guohua，YU Zhaofen，WANG Xuemei |
| 关键字： | 甘草；雷公藤甲素；雷公藤内酯酮；内源代谢物；大鼠 |
| KEYWORDS： | Glycyrrhiza uralensis； Triptolide； Triptonide； Endogenous metabolite； Rats |
| 阅读数： | 369 次 |
| 本月下载数： | 2 次 |
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