改进HPLC法测定注射用丹参多酚酸盐中丹酚酸B的含量
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篇名: 改进HPLC法测定注射用丹参多酚酸盐中丹酚酸B的含量
TITLE:
摘要: 目的:改进注射用丹参多酚酸盐中丹酚酸B含量的测定方法。方法:采用高效液相色谱法。色谱柱为Hypersil ODS C18,流动相为甲醇-乙腈-甲酸-水(30 ∶ 10 ∶ 1 ∶ 59,V/V/V/V),流速为1.0 ml/min,检测波长为286 nm,柱温为25 ℃,进样量为10 μl。结果:丹酚酸B检测质量浓度线性范围为0.1~3.2 mg/ml(r=0.999 6);精密度、稳定性、重复性试验的RSD<2.0%;加样回收率为98.15%~100.23%(RSD=0.58%,n=9)。结论:改进的方法灵敏度更高、重复性更好,适用于测定注射用丹参多酚酸盐中丹酚酸B的含量。
ABSTRACT: OBJECTIVE: To improve the method of content determination of salvianolic acid B in Salvianolate injection. METHODS: HPLC method performed on the column of Hypersil ODS C18 with mobile phase of methanol-acetonitrile-methanoic acid-water (30 ∶ 10 ∶ 1 ∶ 59,V/V/V/V) at a flow rate of 1.0 ml/min, detection wavelength was 286 nm, column temperature was 25 ℃, and injection volume was 10 μl. RESULTS: The linear range of salvianolic acid B was 0.1-3.2 mg/ml(r=0.999 6);RSDs of precision, stability and reproducibility tests were lower than 2%; recovery was 98.15%-100.23%(RSD=0.58%,n=9). CONCLUSIONS: The improved method is more sensitive and reproducible, and suitable for the content determination of salvianolic acid B in Salvianolate injection.
期刊: 2016年第27卷第36期
作者: 吴妍,耿魁魁,邓晓媚,马旖旎,陈辰,刘圣
AUTHORS: WU Yan,GENG Kuikui,DENG Xiaomei,MA Yini,CHEN Chen,LIU Sheng
关键字: 高效液相色谱法;注射用丹参多酚酸盐;丹酚酸B;含量测定
KEYWORDS: HPLC; Salvianolate injection; Salvianolic acid B; Content determination
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