改进HPLC法测定注射用多种维生素(12)中维生素B12的含量
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篇名: 改进HPLC法测定注射用多种维生素(12)中维生素B12的含量
TITLE:
摘要: 目的:改进高效液相色谱法测定注射用多种维生素(12)中维生素B12的含量。方法:采用高效液相色谱法。色谱柱为Kromasil 100-5 C18,流动相为水(用10%稀磷酸调节pH为4.5)-乙腈(梯度洗脱), 流速为1.2 ml/min,检测波长为361 nm,柱温为35 ℃,进样量为50 μl。结果:维生素B12检测进样量线性范围为15.03~45.09 ng(r=0.999 9);精密度、稳定性、重复性试验的RSD<1%;回收率为99.31%~100.90%(RSD=0.42%,n=9)。结论:该方法简便、准确、灵敏度高、重复性好,可用于注射用多种维生素(12)中维生素B12的含量测定。
ABSTRACT: OBJECTIVE: To improve the method for the content determination of vitamin B12 in Multivitamin for injection (12). METHODS: HPLC was performed on the column of Kromasil 100-5 C18 with mobile phase of water (adjusted to pH4.5 with 10% diluted phosphoric acid)-acetonitrile (gradient elution) at a flow rate of 1.2 ml/min, the detection wavelength was 361 nm, column temperature was 35 ℃, and injection volume was 50 μl. RESULTS: The linear range of vitamin B12 was 15.03-45.09 ng (r=0.999 9); RSDs of precision, stability and reproducibility tests were no lower than 1%; recovery was 99.31%-100.90% (RSD=0.42%,n=9). CONCLUSIONS: The method is simple, accurate, sensitive, reproducible, and can be used for the content determination of vitamin B12 in Multivitamin for injection (12).
期刊: 2016年第27卷第36期
作者: 徐家根,刁岩忠
AUTHORS: XU Jiagen,DIAO Yanzhong
关键字: 注射用多种维生素(12);维生素B12;含量测定;高效液相色谱法;方法改进
KEYWORDS: Multivitamin for injection (12); Vitamin B12; Content determination; HPLC; Method improvement
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