：提取、鉴定陵水暗罗根提取物中挥发性成分，并评价其体外生物活性。方法：采用超临界CO2萃取法提取陵水暗罗

根中的挥发性化学成分，气相色谱-质谱联用（GC-MS）法分析其化学成分组成；以0（空白对照）、5、10、20、30、40、50 μg（生药）/ml

提取物培养人肝癌Huh-7 细胞24 h 后，采用MTT法测定其对细胞的抑制作用，计算相对细胞活力和半数抑制浓度（IC50）；分别采

用纸片琼脂扩散法和肉汤稀释法测定其对金黄色葡萄球菌、耐甲氧西林金黄色葡萄球菌和粪肠球菌等菌株的体外抗菌活性。结

果：从陵水暗罗根超临界CO2萃取物中分离鉴定了40 个化合物，主要成分为（Z，Z，Z）-9，12，15-octa-decatrien-1-ol、甘油、肉桂醛、棕

榈酸和丁香酚等；与空白对照组比较，陵水暗罗根提取物在5 μg（生药）/ml 以上质量浓度时对细胞生长有显著抑制作用（P＜

0.05），且随着质量浓度的增加抑制作用增强，IC50值为5.2 μg（生药）/ml；两种抑菌试验均未检测出其具有抑菌活性。结论：超临界

CO2萃取法和GC-MS法能有效提取和鉴定陵水暗罗根超临界CO2萃取物的挥发性成分；提取物表现出抑制细胞活力的作用，但无

抗菌活性。

OBJECTIVE：To extract and identify the volatile components of the roots of Polyalthia nemoralis，and to evaluate

its biological activity in vitro. METHODS：Supercritical CO2 extraction（SFE-CO2）was performed to extract volatile compounds

from the roots of P. nemoralis，and the volatile components were separated and determined by GC-MS. Human hepatic cancer

Huh-7 cells were cultured with 0（blank control），5，10，20，30，40 and 50 μg（medicinal material）/ml extract for 24 h，and then

MTT assay was used to detect the inhibitory effect of the extracts on cells. Relative cell viability and IC50 were calculated. The anti-

bacterial activities of extract to Staphylococcus aureus，Methicillin-resistant Staphylococcus aureus，Enterococcus faecalis and other

strain were determined by Kirby-bauer method and broth dilution method. RESULTS：Forty compounds were identified from the

SFE-CO2 extracts from the roots of P. nemoralis. The main constituents were（Z，Z，Z）-9，12，15-octa-decatrien-1-ol，glycerin，cinnamaldehyde，

n-hexadecanoic acid and eugenol. Compared with blank control group，SFE-CO2 extracts from the roots of P. nemoralis

5 μg（medicinal material）/ml and above showed significant inhibitory effect on cell growth（P＜0.05），and the inhibitory effect was

strengthened as the concentration of extracts increased，IC50 values was 5.2 μg（medicinal material）/ml. However，the supercritical

extract didn’t showed antibacterial activity against three microorganisms in 2 kinds of antibacterial tests. CONCLUSIONS：

SFE-CO2 and GC-MS method can effectively extract and identify volatile components of the roots of P. nemoralis，and supercritical

extracts can inhibit the viability of cells but have no antibacterial activity.