目的：研究蛋白激酶B（Akt）抑制剂MK-2206 对肺腺癌A549 细胞增殖和凋亡的影响。方法：MTT法检测0（空白对照）、

0.5、1、2.5、5、10、20、30 μmol/L MK-2206 处理A549 细胞24 h 后细胞的光密度；以0（空白对照）、5、10、20 μmol/L MK-2206 处理

A549 细胞24 h，倒置相差显微镜观察细胞形态变化，流式细胞术分析细胞周期分布和凋亡率，免疫印迹法检测细胞周期相关蛋白

Cyclin D1、p21、p27 和细胞凋亡相关蛋白分裂的多聚腺苷二磷酸核糖聚合酶（cf-PARP）、分裂的半胱氨酸天冬氨酸蛋白酶3

（cf-caspase-3）、B淋巴细胞瘤2（Bcl-2）和Bax 的表达。结果：与空白对照比较，MK-2206 处理后A549 细胞的光密度降低；细胞缩

小、呈空泡状；主要阻滞于G0/G1期，细胞中p21、p27 蛋白表达增强，Cyclin D1 蛋白表达减弱；细胞凋亡率增加，细胞内cf-PARP、

cf-caspase-3、Bax 表达增强，Bcl-2 表达减弱。各效应均呈浓度依赖性（P＜0.05 或P＜0.01）。结论：MK-2206 可抑制A549 细胞增

殖，并通过激活caspase-3、下调Bcl-2 和上调Bax蛋白的表达诱导其凋亡。

OBJECTIVE：To study the effects of Akt inhibitor MK-2206 on the proliferation and apoptosis of lung adenocarcinoma

A549 cells. METHODS：The optical density of A549 cells was detected by MTT assay after treated with 0（blank control），0.5，

1，2.5，5，10，20 and 30 μmol/L MK-2206 for 24 h；after pretreatment with 0（blank control），5，10 and 20 μmol/L MK-2206 for 24

h，morphological changes of A549 cells were observed with inverted microscope. Cell cycle and apoptosis rate were analyzed by

flow cytometry. Western blot was used to detect the expression of cell cycle-related proteins Cyclin D1，p21 and p27 and apoptosis-

related protein PARP（cf-PARP），cf-caspase-3，Bcl-2 and Bax. RESULTS：Compared with blank control，the optical density of

A549 cells decreased，cells shrank and presented vesicular state after treatment of MK-2206；A549 cells arrested in G0/G1 stage，

the protein expression of p21 and p27 strengthened while that of Cyclin D1 decreased；the apoptotic rate of cells increased，the expression

of cf-PARP，cf-caspase-3 and Bax in cells increased while that of Bcl-2 decreased. All reponse were in concentration-dependant

manner （P＜0.05 or P＜0.01）. CONCLUSIONS：MK-2206 can inhibit the proliferation of A549 cells，and induce the

apoptosis of A549 cells by adjusting the expression of activating caspase-3，down-regulating Bcl-2 and up-regulating Bax.