目的：优化益气固本颗粒提取液的澄清工艺。方法：以多糖、毛蕊异黄酮葡萄糖苷含量和干膏的转移率为评价指标，考

察澄清剂分别为ZTC1+1Ⅱ型、壳聚糖、95％乙醇时的澄清效果；再以料液比、澄清剂加入量、静置时间为考察因素，对益气固本颗粒

提取液的澄清工艺条件设计正交试验进行优化，并进行验证试验。结果：以ZTC1+1Ⅱ型为澄清剂时有效成分转移率更高；最优澄

清工艺为料液比1 ∶2，ZTC1+1Ⅱ型A液用量5％、B液用量10％，静置时间5 h。验证试验中3 次试验干膏转移率分别为71.54％、

70.98％、69.21％，多糖转移率分别为82.55％、81.78％、82.15％，毛蕊异黄酮葡萄糖苷转移率分别为91.92％、92.34％、91.58％

（RSD均≤1.72％，n＝3）。结论：优选的澄清工艺有效、稳定、可行，可用于益气固本颗粒提取液的澄清纯化。

OBJECTIVE：To optimize the purification process of the extract from Yiqi guben granules. METHODS：The purification

effect of the process was investigated with transfer rate of polysaccharide，calycosin glucoside and dry paste as evaluation indexes，

using ZTC1 + 1 type Ⅱ，and shell poly sugar and 95％ ethanol as clarifying agents. The purification process of the extract

from Yiqi guben granules was optimized by orthogonal test using the ratio of material to liquid，the amount of clarifying agent and

standing time as factor. The validation test was conducted. RESULTS：Selecting ZTC1+1 type Ⅱ as a clarifying agent，the best transfer

rate of effective component had been obtained；optimal purification process was as follows as the ratio of material to liquid 1 ∶2，

the ratio of ZTC1 + 1 type ⅡA liquid 5％，the ratio of B liquid 10％，standing time of 5 h. The results of verification test showed

transfer rates of dry paste in 3 tests were 71.54％ ，70.98％ ，69.21％ ，respectively；those of polysaccharide were 82.55％ ，

81.78％，82.15％，respectively；those of calycosin glucoside were 91.92％，92.34％，91.58％，respectively（all RSD≤1.72％，

n＝3）. CONCLUSIONS：The optimized purification process is effective，stable and practical，and can be used for the purification

of the extract from Yiqi guben granules.