目的：优化茯苓苦瓜胶囊中粗多糖和总皂苷的综合提取工艺。方法：依据单因素试验的结果，分别以料液比、提取时间、

提取次数为响应因素，以粗多糖和总皂苷得率为响应值，采用响应面法中的Box-Behnken 设计优化茯苓苦瓜胶囊中粗多糖和总皂

苷的综合提取工艺并进行验证。结果：最优综合提取工艺为料液比1 ∶12、提取1.4 h、提取2 次。在此工艺下，验证试验中实测粗多

糖得率为14.44％、总皂苷得率为1.33％（RSD 分别为0.14％、0.75％，n＝3），与预测值14.69％、1.36％接近，偏差分别为1.70％、

2.21％。结论：实测值与预测值接近，表明采用响应面法优化后得到的综合提取工艺参数可用于茯苓苦瓜胶囊中粗多糖和总皂苷

的提取。

OBJECTIVE：To optimize the comprehensive extraction technology of crude polysaccharide and total saponins from

Fuling kugua capsule. METHODS：Based on single factor test，using liquid ratio，extraction time and extraction times as response

factors，the parameters of extraction technology of crude polysaccharides and total saponins from Fuling kugua capsule were optimized

by using Box-Behnken design of response-surface method. RESULTS：The optimal extraction technology was as follows as

liquid ratio of 1 ∶12，extraction time for 1.4 h，extraction times for 2 times. Under this technology，the yield of crude polysaccharide

was 14.44％（RSD＝0.14％，n＝3），and that of total saponins was 1.33％（RSD＝0.75％，n＝3）. They were close to predicted

value 14.69％ and 1.36％，and the deviation were 1.70％ and 2.21％. CONCLUSIONS：Measured value is close to predicted

value，which indicates comprehensive extraction parameters optimized by response-surface methodology can be sued for the extraction

of crude polysaccharide and total saponins from Fuling kugua capsule.