益气温阳护卫汤对支气管哮喘大鼠的改善作用及机制-中国药房

益气温阳护卫汤对支气管哮喘大鼠的改善作用及机制

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篇名：	益气温阳护卫汤对支气管哮喘大鼠的改善作用及机制
TITLE：	Effects and mechanisms of action of Yiqi wenyang huwei decoction in improving bronchial asthma in rats
摘要：	目的基于Toll样受体4（TLR4）/髓系分化初级反应蛋白88（MyD88）/核因子κB（NF-κB）信号通路，探讨益气温阳护卫汤（YQWY）对支气管哮喘（BA）大鼠气道炎症及重塑的改善作用及潜在机制。方法将雄性SD大鼠随机分为正常组、模型组、地塞米松组（阳性对照，0.5mg/kg）和YQWY低、中、高剂量组（5、10、20g/kg，按生药量计），每组8只。除正常组外，其余各组大鼠均采用卵清蛋白双重致敏联合雾化激发的方式构建BA模型；各组大鼠于实验第14～34天，在雾化激发前1h灌胃相应药液或生理盐水1次。末次雾化后24h，对各组大鼠进行哮喘体征评分，检测其血清免疫球蛋白E（IgE）水平，支气管肺泡灌洗液中炎症因子（白细胞介素4、5、13和肿瘤坏死因子α）水平和炎症细胞（白细胞、嗜酸性粒细胞、中性粒细胞、淋巴细胞、单核细胞及嗜碱性粒细胞）数量，观察其肺组织病理改变，检测其肺组织中TLR4、MyD88、NF-κBmRNA及TLR4、MyD88、NF-κBp65、磷酸化NF-κBp65蛋白的表达情况。结果与模型组相比，各药物组大鼠肺组织炎症细胞浸润、胶原纤维异常沉积、杯状细胞增生等病理改变均有不同程度缓解；哮喘体征评分（YQWY低剂量组除外），IgE和炎症因子水平（YQWY中剂量组白细胞介素5除外），炎症细胞数量（YQWY低剂量组单核细胞、嗜碱性粒细胞除外），TLR4、MyD88、NF-κBmRNA的表达，以及TLR4、MyD88、NF-κBp65、磷酸化NF-κBp65蛋白的表达[YQWY低剂量组MyD88、NF-κBp65蛋白（Westernblot法）除外]均显著降低或下调（P＜0.05或P＜0.01）。结论YQWY能够减轻BA大鼠的哮喘样表现，改善其气道炎症及重塑；上述作用可能与该方抑制TLR4/MyD88/NF-κB信号通路异常激活有关。
ABSTRACT：	OBJECTIVE To investigate the effects and potential mechanism of Yiqi wenyang huwei decoction （YQWY） in improving airway inflammation and remodeling in rats with bronchial asthma （BA） based on the Toll-like receptor 4 （TLR4）/myeloid differentiation primary response protein 88 （MyD88）/nuclear factor-κB （NF-κB） signaling pathway. METHODS Male SD rats were randomly divided into the normal group， the model group， the dexamethasone group （positive control， 0.5 mg/kg）， and YQWY low-， medium- and high-dose groups （5， 10， 20 g/kg， calculated by the crude drug）， with 8 rats in each group. Except for the normal group， rats in all other groups were sensitized twice with ovalbumin combined with aerosol challenge to establish a BA model. From day 14 to day 34 of the experiment， the rats in each group were administered the corresponding drug solution or normal saline intragastrically， once a day， 1 hour before aerosol challenge. At 24 hours after the final aerosol challenge， asthma symptom scores were assessed， serum levels of immunoglobulin E （IgE） were measured， and the levels of inflammatory cytokines （interleukin-4， interleukin-5， interleukin-13 and tumor necrosis factor-α） and the numbers of inflammatory cells （white blood cell， eosinophil， neutrophil， lymphocyte， monocyte and basophil） in bronchoalveolar lavage fluid were determined. Pathological changes in lung tissue were observed. The mRNA expressions of TLR4， MyD88 and NF-κB， as well as the protein expressions of TLR4， MyD88， NF-κB p65 and phosphorylated NF-κB p65 in lung tissue， were detected. RESULTS Compared with the model group， the pathological changes， such as inflammatory cell infiltration， abnormal deposition of collagen fibers， and goblet cell hyperplasia in the lung tissue of rats in each drug group， were alleviated to varying degrees. The asthma symptom scores （except for the YQWY low-dose group）， the levels of IgE and inflammatory cytokines （except for interleukin-5 in the YQWY medium-dose group）， the number of inflammatory cells （except for monocyte and basophil in the YQWY low-dose group）， the mRNA expression of TLR4， MyD88 and NF-κB， as well as the protein expressions of TLR4， MyD88， NF-κB p65 and phosphorylated NF-κB p65 （except for MyD88 and NF-κB p65 proteins in the YQWY low-dose group as detected by Western blo t） were all significantly reduced or down-regulated （ P ＜0.05 or P ＜0.01）. CONCLUSIONS YQWY can alleviate asthma-like manifestations in BA rats and improve their airway inflammation and remodeling； these effects may be related to the formula’s inhibition of the abnormal activation of the TLR4/MyD88/NF-κB signaling pathway.
期刊：	2026年第37卷第10期
作者：	杨云青；谢建宇；唐薇；叶超；喻强强；孙朋；杨玉萍；余建玮
AUTHORS：	YANG Yunqing，XIE Jianyu，TANG Wei，YE Chao，YU Qiangqiang，SUN Peng，YANG Yuping，YU Jianwei
关键字：	益气温阳护卫汤；支气管哮喘；气道炎症；气道重塑；TLR4/MyD88/NF-κB信号通路
KEYWORDS：	Yiqi wenyang huwei decoction； bronchial asthma； airway inflammation； airway remodeling； TLR4/MyD88/NF-κB
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