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基于肝阳上亢高血压大鼠模型研究某介类中药药对不同煎煮工艺的等效性
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| 篇名： | 基于肝阳上亢高血压大鼠模型研究某介类中药药对不同煎煮工艺的等效性 |
| TITLE： | Study on the equivalence of different decoction processes for a shell-based traditional Chinese medicine drug pair based on rat model of hypertension with liver-yang hyperactivity |
| 摘要： | 目的 基于肝阳上亢高血压大鼠模型，研究牡蛎-石决明-珍珠母药对不同煎煮工艺的等效性。方法采用电感耦合等离子体质谱（ICP-MS）法比较牡蛎-石决明-珍珠母药对饮片先煎与粉末直煎水煎液无机元素的溶出差异，以6只SD大鼠为正常组，利用自发性高血压大鼠灌胃附子汤6周复制肝阳上亢型高血压模型，将48只造模成功大鼠随机分为模型组、阳性对照组[卡托普利，8mL/（kg·d）]，以及饮片先煎和粉末直煎低、中、高剂量组[各2.02、4.05、8.10mL/（kg·d）]，每组6只。各组大鼠灌胃相应药物或等体积纯水，每天1次，连续2周。末次给药后，观察各组大鼠暴躁易怒程度、面部温度、压力疼痛阈值、血压和胸主动脉病理变化，并检测血清一氧化氮（NO）和血浆血管紧张素Ⅱ（AngⅡ）、肾素（renin）和醛固酮（ALD）水平。结果ICP-MS分析结果显示，粉末直煎水煎液与饮片先煎水煎液中Li、Na、Mg、Ca、Mn、Ga、Sr、Mo、Cd、Sn、Sb含量比较差异有统计学意义（P＜0.05）；而P、Cr、Fe、Ni、Zn、Hg、Tl、Pb均未检出。动物实验表明，给药2周后，与模型组比较，各给药组大鼠面部温度、血压均降低，压力疼痛阈值均升高，血浆AngⅡ、renin、ALD及血清NO水平均降低，中膜厚度显著变薄，以上指标大部分差异有统计学意义（P＜0.05或P＜0.01或P＜0.001）；病理观察显示胸主动脉病理损伤改善。结论牡蛎-石决明-珍珠母药对采用粉末直煎工艺在显著促进Ca、Mg、Sr等关键元素溶出的同时，未伴随有害元素溶出量的增加；在改善肝阳上亢证候、降低血压及保护血管内皮等方面，与传统饮片先煎工艺效果相当，部分指标表现更优。 |
| ABSTRACT： | OBJECTIVE To investigate the equivalence of different decoction processes based on rat model of hypertension with liver-yang hyperactivity. METHODS Inductively coupled plasma mass spectrometry （ICP-MS） was used to compare the dissolution differences of inorganic elements in the powder-directly-decocted decoction versus the pieces-decocted-first decoction of Ostreae Concha- Haliotidis Concha- Margaritifera Concha drug pair. Six SD rats were included in the normal group. The spontaneously hypertensive rats were given Aconite decoction for six weeks to induce the hypertension model with liver-yang hyperactivity. After successful modeling， 48 rats were randomly divided into the model group， the captopril group [positive control， 8 mL/（kg·d） ] ， as well as low-， medium-， and high-dose groups of pieces decocted first or directly powder decocted [2.02， 4.05， 8.10 mL/（kg·d） ] ， with 6 rats in each group. Each group received the corresponding drug or equal volume of pure water intragastrically， once a day， for two consecutive weeks. After the last administration， the degree of irritability， facial temperature， pressure pain threshold， blood pressure， and pathological changes of the thoracic aorta were observed in each group. Serum nitric oxide （NO） and plasma angiotensin Ⅱ （Ang Ⅱ）， renin， and aldosterone （ALD） levels were also measured. RESULTS ICP-MS analysis results showed statistically significant differences in the contents of macroelements Li， Na， Mg， Ca， Mn， Ga， Sr， Mo， Cd， Sn， and Sb， between the powder-directly-decocted decoction and the pieces-decocted-first decoction （ P ＜0.05） ，the elements P， Cr， Fe， Ni， Zn， Hg， Tl， and Pb were not detected in either decoction. Animal experiments showed that after two weeks of administration， compared with the model group， the facial temperature， and blood pressure decreased in all treatment groups， while the pressure pain threshold increased； plasma levels of Ang Ⅱ， renin and ALD， as well as the serum level of NO were all decreased， and thoracic aortic media thickness was significantly reduced， most of the differences in the above indicators were statistically significant （ P ＜0.05 or P ＜0.01 or P ＜0.001）. Pathological observation showed improvement in thoracic aortic pathological injury. CONCLUSIONS The powder-directly-decocted process for the Ostreae Concha- Haliotidis Concha- Margaritifera Concha drug pair significantly promotes the dissolution of key elements such as Ca， Mg， and Sr without increasing the dissolution of harmful elements. It is equivalent to the traditional pieces-decocted-first in alleviating liver-yang hyperactivity syndrome， lowering blood pressure， and protecting the vascular endothelium， and even shows better performance in some indicators. |
| 期刊： | 2026年第37卷第10期 |
| 作者： | 李明露；崔雨晗；吴翻；韩可昕；吴丽；方祝元；束雅春 |
| AUTHORS： | LI Minglu，CUI Yuhan，WU Fan，HAN Kexin，WU Li，FANG Zhuyuan，SHU Yachun |
| 关键字： | 介类中药；肝阳上亢；电感耦合等离子体质谱；牡蛎；石决明；珍珠母；药对 |
| KEYWORDS： | shell-based traditional Chinese medicines； liver-yang hyperactivity； inductively coupled plasma mass spectrometry； |
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