四味颗粒改善三阴性乳腺癌的活性物质筛选及作用研究-中国药房

四味颗粒改善三阴性乳腺癌的活性物质筛选及作用研究

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篇名：	四味颗粒改善三阴性乳腺癌的活性物质筛选及作用研究
TITLE：	Screening of bioactive compounds and ameliorative effects of Siwei granules on triple-negative breast cancer
摘要：	目的筛选四味颗粒改善三阴性乳腺癌（TNBC）的活性物质，并探讨其抗TNBC作用。方法采用网络药理学方法，筛选四味颗粒活性成分、对应靶点及TNBC相关靶点，构建“成分-靶点”互作调控网络，并进行基因本体（GO）、京都基因和基因组数据库富集分析。以MDA-MB-231、4T1细胞为对象，筛选四味颗粒抗TNBC的活性物质；以MDA-MB-231细胞为对象，探讨活性物质单用或联用对细胞凋亡、迁移、周期、活性氧（ROS）生成以及富集通路相关蛋白表达的影响。结果通过网络药理学方法共获得活性成分67个，成分靶点与TNBC靶点的交集靶点131个；核心关键作用靶点包括磷脂酰肌醇-4，5-二磷酸3-激酶催化亚基α等6个；交集靶点富集于增殖、含磷基团转移酶复合物、蛋白酪氨酸激酶活性等GO功能，以及磷脂酰肌醇3激酶（PI3K）/蛋白激酶B（Akt）、ROS等信号通路。通过细胞实验共筛选得到4个活性物质，其中熊果酸对2种细胞的半数抑制浓度为25μmol/L，甘草查尔酮A、木犀草素、芦荟大黄素（仅对4T1细胞有作用）对2种细胞的半数抑制浓度均为50μmol/L；4种物质单用（20μmol/L）或联用（各10μmol/L），可显著升高MDA-MB-231细胞的凋亡率（仅联合组）、相对ROS水平（仅联合组）和G2期细胞比例（仅木犀草素组、甘草查尔酮A组和联合组），并显著降低G1期细胞比例（仅甘草查尔酮A组和联合组）、划痕愈合率和PI3K（芦荟大黄素组除外）、Akt（芦荟大黄素组除外）、哺乳动物雷帕霉素靶蛋白的磷酸化水平（P＜0.05）。结论四味颗粒抗TNBC的活性物质可能为熊果酸、甘草查尔酮A、木犀草素、芦荟大黄素，这些物质可不同程度地抑制TNBC细胞增殖和迁移，促进其凋亡，阻滞细胞周期于G2期，诱导ROS的生成，并抑制PI3K/Akt信号通路相关蛋白的磷酸化。
ABSTRACT：	OBJECTIVE To screen for bioactive compounds in Siwei granules against triple-negative breast cancer （TNBC）， and explore their anti-TNBC effects.METHODS Network pharmacology was applied to screen bioactive compounds of Siwei granules， corresponding targets and TNBC-related targets. A “component-target” interaction regulatory network was constructed， followed by Gene Ontology （GO） and Kyoto Encyclopedia of Genes and Genomes （KEGG） enrichment analyses. Using MDA-MB-231 and 4T1 cells as subjects， bioactive compounds from Siwei granules against TNBC were screened. Using MDA-MB-231 cells as the subject， the effects of these bioactive compounds， either alone or in combination， on cell apoptosis， migration， cell cycle， reactive oxygen species （ROS） generation， and the expression of proteins related to enriched pathways were explored.RESULTS A total of 67 bioactive compounds were identified by network pharmacology， with 131 overlapping targets between the compound targets and TNBC targets. Six core key targets were identified， including the phosphatidylinositol-4，5-bisphosphate 3-kinase catalytic subunit alpha， etc. The overlapping targets were enriched in GO functions such as proliferation， phosphotransferase complexes， and protein tyrosine kinase activity， as well as in signaling pathways including phosphoinositide 3-kinase （PI3K）/protein kinase B （Akt） and ROS. A total of 4 bioactive compounds were identified by cell experiments. Among them， the half-maximal inhibitory concentration （IC 50 ） of ursolic acid for both cell lines was 25 μmol/L， while that of licochalcone A， luteolin， and aloe-emodin （for 4T1 cells only） was 50 μmol/L for both cell lines. When administered alone （all 20 μmol/L） or in combination （10 μmol/L each）， the four compounds significantly increased the apoptosis rate in MDA-MB-231 cells （only in the combination group only）， relative ROS levels （only in the combination group） and the proportion of cells in the G 2 phase （only in the luteolin group， licochalcone A group， and combination group）， while they significantly reduced the proportion of cells in the G 1 phase （only in the licochalcone A group and the combination group）， scratch healing rate， and the phosphorylation levels of PI3K （except in the aloe-emodin group）， Akt （except in the aloe-emodin group）， and the mammalian target of rapamycin （ P ＜0.05）.CONCLUSIONS The bioactive compounds in Siwei granules responsible for its anti-TNBC activity may be ursolic acid， licochalcone A， luteolin， and aloe-emodin； these compounds can， to varying degrees， inhibit TNBC cell proliferation and migration， promote apoptosis， arrest the cell cycle in the G 2 phase， induce ROS production， and inhibit the phosphorylation the related proteins of the PI3K/Akt signaling pathway.
期刊：	2026年第37卷第20期
作者：	程林；王晓雯；翁邦碧；黄维
AUTHORS：	CHENG Lin，WANG Xiaowen，WENG Bangbi，HUANG Wei
关键字：	四味颗粒;三阴性乳腺癌;活性物质;网络药理学
KEYWORDS：	Siwei granule;triple-negative breast cancer;bioactive compounds;Network pharmacology
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