Chiralcel OJ-H手性色谱柱-HPLC法同时分离4种H1受体拮抗药的对映体
x

请在关注微信后,向客服人员索取文件

篇名: Chiralcel OJ-H手性色谱柱-HPLC法同时分离4种H1受体拮抗药的对映体
TITLE:
摘要: 目的:建立拆分盐酸西替利嗪、盐酸异丙嗪、特非那定和马来酸非尼拉敏4种H1受体拮抗药对映体的方法。方法:采用高效液相色谱法(HPLC),选择Chiralcel OJ-H手性色谱柱,检测波长为230 nm,柱温为室温,流速为1.0 mL/min,进样量为20 μL;以保留时间、选择因子和分离度为指标,考察流动相中酸碱添加剂(二乙胺)、有机改性剂的种类(乙醇、异丙醇)及其占比、甲醇等对4种药物对映体分离的影响。结果:流动相考察结果为盐酸西替利嗪和盐酸异丙嗪的流动相为正己烷-乙醇(90 ∶ 10,V/V);特非那定的流动相为正己烷-甲醇-乙醇-二乙胺(89 ∶ 9.9 ∶ 1 ∶ 0.1,V/V/V/V);马来酸非尼拉敏对映体的流动相为正己烷-甲醇-乙醇-二乙胺(98 ∶ 2 ∶ 0.5 ∶ 0.1,V/V/V/V)。在此条件下,4种药物的对映体均达到完全分离,分离度分别为4.36、3.76、1.55、1.71。结论:建立的Chiralcel OJ-H手性色谱柱-HPLC法对这4种H1受体拮抗药的选择性良好,可用于其对映体的手性分离。
ABSTRACT: OBJECTIVE: To establish a method for separating of 4 kinds of H1-receptor antagonist enantiomers as cetirizine hydrochloride, promethazine hydrochloride, terfenadine and pheniramine maleate. METHODS: HPLC method was adopted. Chiralcel OJ-H chiral column was selected. Detection wavelength was set at 230 nm; column temperature was room temperature; flow rate was 1.0 mL/min; sample size was 20 μL. Using retention time, selective factors and separation rate as indexes, the influence of acid-base additives (diethylamine), type and ratio of organic modifiers (ethanol, isopropanol), methanol on the separation of 4 kinds of enantiomers in mobile phase were investigated. RESULTS: The results of mobile phase investigation was the mobile phase of cetirizine hydrochloride and promethazine hydrochloride was n-hexane and ethanol (90 ∶ 10, V/V). Mobile phase of terfenadine was n-hexane-methanol-ethanol-diethylamine (89 ∶ 9.9 ∶ 1 ∶ 0.1, V/V/V/V). Mobile phase of pheniramine maleate enantiomers was n-hexane-methanol-ethanol-diethylamine (98 ∶ 2 ∶ 0.5 ∶ 0.1, V/V/V/V). Under the condition, 4 kinds of drug enantiomers were separated completely, with separate rate of 4.36, 3.76, 1.55, 1.71. CONCLUSIONS: Established Chiralcel OJ-H chiral column-HPLC is selective for these 4 kinds of H1-receptor antagonists, and can be used for the chiral separation of the enationmers.
期刊: 2018年第29卷第23期
作者: 李尚颖,陈默,张耀文,隋晓璠,孙苓苓,国梦然
AUTHORS: LI Shangying,CHEN Mo,ZHANG Yaowen,SUI Xiaofan,SUN Lingling,GUO Mengran
关键字: Chiralcel OJ-H手性色谱柱;高效液相色谱法;H1受体拮抗药;盐酸西替利嗪;盐酸异丙嗪;特非那定;马来酸非尼拉敏;对映体
KEYWORDS: Chiralcel OJ-H chiral column; HPLC; H1-receptor antagonists; Cetirizine hydrochloride; Promethazine hydrochloride; Terfenazine; Pheniramine maleate; Enantiomer
阅读数: 556 次
本月下载数: 4 次

* 注:未经本站明确许可,任何网站不得非法盗链资源下载连接及抄袭本站原创内容资源!在此感谢您的支持与合作!