青稞多糖对糖尿病模型小鼠的降血糖作用及机制研究
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篇名: 青稞多糖对糖尿病模型小鼠的降血糖作用及机制研究
TITLE: Study on Hypoglycemic Effect of Hordeum vulgare Polysaccharide on Diabetes Mellitus Model Mice and Its Mechanism
摘要: 目的:研究青稞多糖(HVP)对糖尿病模型小鼠的降血糖作用及机制。方法:对小鼠腹腔注射链脲佐菌素(120mg/kg)以复制糖尿病模型。将造模成功的小鼠随机分为模型组、二甲双胍组(阳性对照,200mg/kg)和HVP高、中、低剂量组(300、150、75mg/kg),另设空白对照组,每组10只。各给药组灌胃相应药物,空白对照组和模型组小鼠灌胃等量水,每天1次,连续30天。给药10、20、30天时测定小鼠的空腹血糖(FBG);末次FBG测定后,小鼠腹腔注射10%葡萄糖溶液(2g/kg),于注射后30、120min时测定糖耐受量(GTT)曲线下面积;测定小鼠血清中胰岛素水平和肝组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)的含量;采用苏木精-伊红染色法观察小鼠胰腺组织病理学变化。结果:与空白对照组比较,模型组小鼠FBG、GTT曲线下面积和肝组织中MDA含量均显著升高或增加(P<0.01);血清中胰岛素水平和肝组织中SOD、GSH-Px含量均显著降低(P<0.01);胰腺组织损伤严重,形态不完整,有明显的空泡。与模型组比较,HVP高剂量组小鼠FBG、GTT曲线下面积和肝组织中MDA含量均显著降低或减少(P<0.05或P<0.01),血清中胰岛素含量和肝组织中SOD、GSH-Px含量均显著升高(P<0.05或P<0.01),HVP低、中剂量组上述指标部分改善,差异具有统计学意义(P<0.05或P<0.01);胰腺组织损伤有所减轻,形态较为完整,胰岛细胞排列紧密,空泡减少。结论:HVP可降低糖尿病模型小鼠的血糖水平,升高胰岛素水平并减轻胰腺组织损伤;该作用机制可能与其抗氧化作用有关。
ABSTRACT: OBJECTIVE:To study the h ypoglycemic effect of Hordeum vulgare polysaccharide(HVP)on diabetes mellitus model mice and its mechanism. METHODS :The mice were given intraperitoneal injection of streptozotocin (120 mg/kg)to induce diabetes mellitus model. After modeling ,the mice were randomly divided into model group ,metformin group (positive control , 200 mg/kg),HVP high-dose ,medium-dose and low-dose groups (300,150 and 75 mg/kg),with 10 mice in each group. Blank control group was established additionally. Administration groups were given relevant medicine intragastrically ;blank control group and model group were given constant volume of water intragastrically ,once a day ,for consecutive 30 days. The levels of fasting blood glucose (FBG)were determined after 10,20,30 days of administration. After last FBG test ,mice were intraperitoneally injected with 10% glucose solution (2 g/kg),then the area under the glucose tolerance (GTT)curve was measured at 30 and 120 min after injection. The serum levels of insulin ,the content of superoxide dismutase (SOD),glutathione peroxidase (GSH-Px), malondialdehyde(MDA)in liver tissue were detected. Pancreatic morphology of mice were detected by HE staining. RESULTS : Compared with blank control group ,the FBG ,area under GTT curve and MDA contnet in liver tissue were increased significantly in model group (P<0.01),while serum levels of insulin ,SOD and GSH-Px contents in liver tissue were decreased significantly (P<0.01);the pancreatic tissue was seriously damaged with incomplete morphology and obvious vacuoles. Compared with model group,FBG,area under GTT curve ,MDA content in liver Δ 基金项目:国家自然科学基金资助项目(No.81473179) tissue were decreased significantly in HVP high-dose group *主管药师 。研究方向 :临床药学 。电话:0510-85351490。E- mail:Wangshengya_helen@126.com (P<0.05 or P<0.01),serum content of insulin ,the content # 通信作者 :副主任药师 。研究方向 :医院药学 。电话:0510- of SOD and GSH-Px in liver tissue were increased 85351490。E-mail:3103221944@qq.com significantly(P<0.05 or P<0.01);above indexes of HVP 中国药房 2021年第32卷第7期 China Pharmacy 2021Vol. 32 No. 7 ·807· low-dose and medium-dose groups were improved partially ,with statistical significance (P<0.05 or P<0.01);the pancreatic tissue damage was alleviated ,the morphology was relatively complete ,the islet cells were closely arranged ,and the vacuoles were reduced. CONLUSIONS :HVP can reduce the level of blood glucose in diabetic model mice ,increase insulin level and relieve pancreatic injury. The mechanism may be related to its antioxidation.
期刊: 2021年第32卷第07期
作者: 王生亚,薛洁,徐乃玉,张真庆,吕栋
AUTHORS: WANG Shengya ,XUE Jie,XU Naiyu,ZHANG Zhenqing ,LYU Dong
关键字: 青稞多糖;糖尿病;抗氧化;小鼠
KEYWORDS: Hordeum vulgare polysaccharide;Diabetes mellitus ;Antioixidation;Mice
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