山苦荬保肝活性部位筛选及其化学成分研究
x

请在关注微信后,向客服人员索取文件

篇名: 山苦荬保肝活性部位筛选及其化学成分研究
TITLE:
摘要: 目的:筛选山苦荬保肝活性部位并对其化学成分进行研究。方法:采用系统溶剂法,以70%乙醇对山苦荬药材进行提取,用不同溶剂对所得提取物进行萃取,分别得石油醚、乙酸乙酯、正丁醇部位和剩余水部位。以对乙酰氨基酚诱导人肝细胞HL-7702复制肝损伤细胞模型,采用MTT法检测上述各部位(40μg/mL,均以生药量计)对损伤细胞的保护作用并筛选活性部位。采用硅胶柱、葡聚糖凝胶柱色谱等分离手段对活性部位进行分离纯化,根据理化性质和波谱(氢谱、碳谱)数据鉴定化合物结构。结果:经山苦荬不同萃取部位作用后,各药物组细胞的存活率均较模型组显著升高(P<0.01),其中正丁醇部位和水部位的活性最强(细胞存活率分别为49.3%、52.2%)。从正丁醇部位中共分离并鉴定出6个化合物,分别为苦荬菜木脂素A(Ⅰ)、芹菜素-7-O-β-D-吡喃葡萄糖醛酸苷甲酯(Ⅱ)、木犀草素7-O-β-D-吡喃葡萄糖醛酸苷甲酯(Ⅲ)、木犀草素-7-O-β-D-葡萄糖苷(Ⅳ)、芹菜素-7-O-β-D-葡萄糖苷(Ⅴ)和木犀草素(Ⅵ)。结论:山苦荬正丁醇部位为该药材的保肝活性部位之一,其活性成分以黄酮类化合物为主。
ABSTRACT: OBJECTIVE: To screen the hepatoprotective active fractions from Ixeris chinensis and study its chemical constituents. METHODS:The petroleum ether,ethyl acetate,n-butanol and residual water fractions from 70% ethanol extract of I.chinensis were extracted by systematic solvent method. Human hepatocytes HL-7702 were induced by acetaminophen to induce liver injury model. MTT method was used to detect the protective effect of the above fractions(40 μg/mL,by the dosage of crude drug)on injured cells,and the active fractions were screened. The active fractions were separated and purified by silica gel column and Sephadex column chromatography. The structure of the compounds were identified by physical and chemical properties and spectral data (hydrogen spectrum,carbon spectrum). RESULTS:After treated with different fractions of I. chinensis,the cell survival rate of each administration group was increased significantly,compared with model group(P<0.01),and the n-butanol and water fractions had the strongest activity (the cell survival rates were 49.3% and 52.2% ,respectively). Six compoundswere isolated from n-butanol fraction and identified as sonchifolignan A(Ⅰ),apigenin-7-O-β-D-glucopyranoside methyl ester(Ⅱ),luteolin-7-O-β-D-glucuronopyranoside methyl ester (Ⅲ),luteolin-7-O-β-D-glucopyranoside (Ⅳ),apigenin-7-O-β-Dglucopyranoside(Ⅴ)and luteolin(Ⅵ). CONCLUSIONS:The n-butanol fraction is regarded as an effective position for protecting liver,and flavonoids are the main active omponents.KEYWORDS Ixeris chinensis;Hepatoprotective activi
期刊: 2021年第32卷第09期
作者: 孙丽君,常福厚,白图雅,王玉华
AUTHORS:
关键字: 山苦荬;保肝活性;活性部位筛选;化学成分;鉴定
KEYWORDS: Ixeris chinensis ;Hepatoprotective activity ;Active fraction screening ;Chemical components ;Identification
阅读数: 279 次
本月下载数: 10 次

* 注:未经本站明确许可,任何网站不得非法盗链资源下载连接及抄袭本站原创内容资源!在此感谢您的支持与合作!