HPLC-多波长切换法同时测定经典名方三化汤中12种成分的含量
x

请在关注微信后,向客服人员索取文件

篇名: HPLC-多波长切换法同时测定经典名方三化汤中12种成分的含量
TITLE: Simultaneous Determination of 12 Components in the Classical Formula Sanhua Tang by HPLC-multi-wavelength Switching Technology
摘要: 目的:建立同时测定经典名方三化汤中柚皮苷、橙皮苷、新橙皮苷、芦荟大黄素、大黄酸、羌活醇、大黄素、和厚朴酚、异欧前胡素、厚朴酚、大黄酚、大黄素甲醚等12种成分含量的方法。方法:采用高效液相色谱(HPLC)-多波长切换法。色谱柱为COS-MOSILC18,流动相为乙腈-0.1%磷酸溶液(梯度洗脱),流速为1.0mL/min,检测波长分别为280nm(柚皮苷、橙皮苷、新橙皮苷)、254nm(芦荟大黄素、大黄酸、大黄酚、大黄素甲醚)、310nm(羌活醇、大黄素、和厚朴酚、异欧前胡素、厚朴酚),柱温为30℃,进样量为10μL。结果:12种成分的分离度均较良好,阴性对照无干扰。柚皮苷、橙皮苷、新橙皮苷、芦荟大黄素、大黄酸、羌活醇、大黄素、和厚朴酚、异欧前胡素、厚朴酚、大黄酚、大黄素甲醚分别在55.4~5540、3.8~380、45.6~4560、1.2~120、2.1~210、2.2~220、2~200、2.4~240、0.8~80、1.2~120、1.7~170、1.1~110μg/mL质量浓度范围内与其峰面积的线性关系良好(R2≥0.9996);检测限分别为0.064、0.024、0.053、0.018、0.020、0.041、0.050、0.091、0.030、0.180、0.028、0.083μg/mL,定量限分别为0.213、0.075、0.174、0.060、0.063、0.138、0.166、0.323、0.130、0.600、0.094、0.275μg/mL;精密度、稳定性(24h)和重复性试验的RSD均小于2%(n=6);平均加样回收率分别为99.54%、99.69%、100.01%、99.93%、100.36%、99.65%、100.03%、100.47%、99.97%、100.68%、99.90%、100.17%,RSD均小于2%(n=6)。结论:本研究所建立的HPLC-多波长切换法操作简便、专属性强、稳定性好,可用于三化汤中柚皮苷、橙皮苷、新橙皮苷等12种成分含量的同时测定。
ABSTRACT: OBJECTIVE:To establish a method for simultaneous determination of 12 components including naringin , hesperidin,neohesperidin,aloe emodin ,rhein,notopterol,emodin,honokiol,isoimperatorin,magnolol,chrysophanol and physcion in classical formula Sanhua tang. METHODS : HPLC-multi-wavelength switching technology was adopted. The determination was performed on COSMOSIL C 18 with mobile phase consisted of acetonitrile- 0.1% phosphoric acid (gradient elution)at the flow rate of 1.0 mL/min. The detection wavelength was 280 nm(naringin,hesperidin,neohesperidin),254 nm (aloe emodin ,rhein,chrysophanol,emodin methyl ether ),310 nm(notopterol,emodin,honokiol,isoimperatorin,magnolol). The column temperature was set at 30 ℃,and the sample size was 10 μL. RESULTS:A total of 12 components were well separated without negative interference. The linear range of naringin ,hesperidin,neohesperidin,aloe emodin ,rhein,notopterol, emodin,honokiol,isoimperatorin,magnolol,chrysophanol and physcion were 55.4-5 540,3.8-380,45.6-4 560,1.2-120, 2.1-210,2.2-220,2-200,2.4-240,0.8-80,1.2-120,1.7-170,1.1-110 μg/mL(R 2≥0.999 6),respectively. The detection limits were 0.064,0.024,0.053,0.018,0.020,0.041,0.050,0.091,0.030,0.180,0.028 and 0.083 μg/mL,respectively. The limits of quantitation were 0.213,0.075,0.174,0.060,0.063,0.138, 0.166,0.323,0.130,0.600,0.094 and 0.275 μ g/mL,respec- 9721004) tively. RSDs of precision ,stability (24 h) and repeatability 2633531778@qq.com tests were all lower than 2%(n=6). Average recoveries were 99.54%,99.69%,100.01%,99.93%,100.36%,99.65%, 100.03%,100.47%,99.97%,100.68%,99.90%,100.17% (all RSDs were lower than 2%,n=6),respectively. CONCLUSIONS :Established HPLC-multi- wavelength switching technology is simple ,specific and stable ,which could be used for the simultaneous determination of 12 components in Sanhua tang as naringin,hesperidin,neohesperidin.
期刊: 2021年第32卷第14期
作者: 薛晴,丛竹凤,向泽栋,柏道鸣,代龙,高鹏
AUTHORS: XUE Qing,CONG Zhufeng ,XIANG Zedong ,BAI Daoming ,DAI Long,GAO Peng
关键字: 三化汤;经典名方;含量测定;高效液相色谱法;多波长切换
KEYWORDS: Sanhua tang ;Classic formula ;Content determination ;HPLC;Multi-wavelength switching
阅读数: 268 次
本月下载数: 4 次

* 注:未经本站明确许可,任何网站不得非法盗链资源下载连接及抄袭本站原创内容资源!在此感谢您的支持与合作!