HPLC波长切换法同时测定经典名方槐花散中5种成分的含量
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篇名: HPLC波长切换法同时测定经典名方槐花散中5种成分的含量
TITLE: Simultaneous Determination of 5 Components in the Classical Formula Huaihua San by HPLC Wavelength Switching Method
摘要: 目的:建立同时测定经典名方槐花散中芦丁、柚皮苷、新橙皮苷、槲皮素和胡薄荷酮等5种成分含量的方法。方法:采用高效液相色谱波长切换法。色谱柱为CosmosilC18,流动相为乙腈-0.05%磷酸溶液(梯度洗脱),检测波长依次为257nm(芦丁)、283nm(柚皮苷、新橙皮苷)、254nm(槲皮素)、252nm(胡薄荷酮),流速为1.0mL/min,柱温为30℃,进样量为10μL。结果:芦丁、柚皮苷、新橙皮苷、槲皮素、胡薄荷酮检测质量浓度的线性范围分别为21.7~2170、46~4600、22.3~2230、0.96~96、2.7~270μg/mL(r均大于0.999);精密度、稳定性(24h)、重复性试验的RSD均小于2%(n=6);平均加样回收率分别为100.70%、99.31%、101.10%、100.03%、99.63%(RSD均小于2%,n=9)。3批槐花散样品中,上述5种成分的含量分别为20.055~22.615、25.557~27.806、11.428~13.250、0.350~0.478、2.372~4.011mg/g。结论:所建含量测定方法操作简便、结果准确、重复性好,可用于槐花散中5种成分的同时测定。
ABSTRACT: OBJECTIVE:To develop a method for simultaneous determination of 5 components in classical formula Huaihua san,including rutin ,naringin,neohesperidin,quercetin and pulegone. METHODS :HPLC wavelength switching method was adopted. The determination was performed on Cosmosil C 18 column with mobile phase consisted of acetonitrile- 0.05% phosphoric acid solution (gradient elution )at the flow rate of 1.0 mL/min. The detection wavelengths were set at 257 nm for rutin ,283 nm for naringin and neohesperidin ,254 nm for quercetin ,252 nm for pulegone ,respectively. The column temperature was set at 30 ℃, and sample size was 10 μL. RESULTS:The linear range was 21.7-2 170 μg/mL for rutin,46-4 600 μg/mL for naringin,22.3- 2 230 μg/mL for neohesperidin,0.96-96 μg/mL for quercetin,2.7-270 μg/mL for pulegone(all r>0.999),respectively. RSDs of precision,stability(24 h)and reproducibility tests were all lower than 2%(n=6). Average recoveries were 100.70%,99.31%, 101.10%,100.03% and 99.63%(all RSD <2%,n=9). Among 3 batches of Huaihua san samples ,the contents of above 5 components were 20.055-22.615,25.557-27.806,11.428-13.250,0.350-0.478,2.372-4.011 mg/g,respectively. CONCLUSIONS : Established method is simple ,accurate and reproducible ,and could be used for the simultaneous determination of 5 components in Huaihua san.
期刊: 2021年第32卷第16期
作者: 向泽栋,薛晴,于海涛,孙志强,李震,刘菊妍,张加余,高鹏,代龙
AUTHORS: XIANG Zedong, XUE Qing,YU Haitao,SUN Zhiqiang,LI Zhen,LIU Juyan,ZHANG Jiayu,GAO Peng,DAI Long
关键字: 槐花散;高效液相色谱波长切换法;芦丁;柚皮苷;新橙皮苷;槲皮素;胡薄荷酮;含量测定
KEYWORDS: Huaihua san ;HPLC wavelength switching method ;Rutin;Naringin;Neohesperidin;Quercetin;Pulegone;
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